Upregulation of relaxin receptors in the PDL by biophysical force
| Autor: | M. S. Kim, S. Y. Lee, J. W. Kim, J. H. Kang, J. S. Moon, Y. H. Moon, H. I. Yoo, S. H. Kim, S. Y. Yang, H. M. Ko, U. Ulziisaikhan |
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| Rok vydání: | 2014 |
| Předmět: |
Vascular Endothelial Growth Factor A
Receptors Peptide Tooth Movement Techniques Periodontal Ligament Blotting Western Matrix metalloproteinase Real-Time Polymerase Chain Reaction Receptors G-Protein-Coupled Rats Sprague-Dawley chemistry.chemical_compound stomatognathic system Downregulation and upregulation Animals Humans Periodontal fiber RNA Messenger Receptor General Dentistry Cells Cultured Relaxin Messenger RNA Interleukin-6 Molar In vitro Rats Up-Regulation Cell biology Vascular endothelial growth factor chemistry Immunology Matrix Metalloproteinase 2 Female Matrix Metalloproteinase 1 Biomarkers |
| Zdroj: | Clinical Oral Investigations. 19:657-665 |
| ISSN: | 1436-3771 1432-6981 |
| DOI: | 10.1007/s00784-014-1276-4 |
| Popis: | We have previously reported that relaxin (Rln) expression from the ovary is upregulated by orthodontic tooth movement. This study was performed to test the hypothesis that Rln family peptides (Rxfps), the G-protein-coupled Rln receptor, is induced in periodontal ligament (PDL) cells to modulate the molecules involved in periodontal tissue remodeling while applying biophysical force. Rats were implanted with orthodontic appliances to investigate changes to Rxfps in vivo. An in vitro biophysical force analysis was performed to measure the level of Rxfp 1 messenger RNA (mRNA) in primary human PDL cells. The levels of Rxfp 2 transcription and translation increased in a time-dependent manner during tooth movement. Rxfp 2 was localized in the PDL by immunofluorescence. In vitro analyses revealed that the level of Rxfp 1 mRNA in PDL cells increased significantly with both compression and tension force. The levels of matrix metalloproteinase (MMP)-1, MMP-2, interleukin-6, and vascular endothelial growth factor mRNA, which are important for periodontal tissue remodeling, also changed under force application and Rln treatment. PDL cells responded to Rln to modulate effector molecules for periodontal tissue remodeling by upregulating Rxfps expression under a biophysical force. Rln and Rxfps may serve as a PDL turnover molecule complex to control orthodontic tooth movement. |
| Databáze: | OpenAIRE |
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