Sedimentation of biliary sludge: effect on composition of gallbladder bile from patients with cholesterol, mixed, or pigment stones
| Autor: | V. Lange, Dieter Jüngst, S Christoph, M. I. Eder, J. F. Miquel, C. Von Ritter, Gustav Paumgartner, R. Del Pozo, E. Frimberger |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Bilirubin Biology Gastroenterology Bile Acids and Salts chemistry.chemical_compound Pigment Cholelithiasis Internal medicine medicine Bile Humans Biliary sludge Bile Pigments Phospholipids Cholesterol Gallbladder Mucin Mucins Proteins medicine.disease Gallbladder bile Endocrinology medicine.anatomical_structure chemistry visual_art visual_art.visual_art_medium Composition (visual arts) Female Ultracentrifugation |
| Zdroj: | Scandinavian journal of gastroenterology. 31(3) |
| ISSN: | 0036-5521 |
| Popis: | Ultracentrifugation of bile has been used extensively to remove insoluble material such as sludge from bile before further studies of cholesterol nucleation. Although it has been recognized that this procedure may affect the composition of gallbladder bile, it has not been studied systematically in different gallstone populations. Therefore, we investigated the concentration of biliary lipids, protein, mucin, and bilirubin before and after ultracentrifugation.Gallbladder bile samples were aspirated during laparoscopic surgery from 66 patients (35 with cholesterol, 16 with mixed, and 15 with pigment stones).Whereas the concentrations of bile acids, phospholipids, protein, and bilirubin in gallbladder bile did not change significantly after ultracentrifugation, cholesterol (20.6 +/- 1.6 to 14.8 +/- 1.2 mmol/l) and mucin concentrations (0.99 +/- 0.2 to 0.67 +/- 0.1 mg/ml) and the cholesterol saturation index (1.68 +/- 0.12 to 1.31 +/- 0.10) decreased significantly in gallbladder bile from patients with cholesterol stones.Sedimentation of biliary sludge may profoundly affect the composition of gallbladder bile, which has to be considered in studies of cholesterol saturation and nucleation. The cholesterol concentration difference between native and ultracentrifuged bile reflects the insoluble crystalline fraction of cholesterol and may be useful for quantitation of the mass of cholesterol crystals in gallstone-associated bile samples. |
| Databáze: | OpenAIRE |
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