Isolation, biochemical characterization and antibiofilm effect of a lectin from the marine sponge Aplysina lactuca
| Autor: | Rômulo Farias Carneiro, Renata Pinheiro Chaves, Mayron Alves de Vasconcelos, Ulisses Pinheiro, Anna Luísa Pereira, Paulo Henrique Pinheiro de Lima, Alexandre Holanda Sampaio, Celso Shiniti Nagano, Edson Holanda Teixeira, Rafael Pereira |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Circular dichroism Staphylococcus aureus Electrospray ionization Carbohydrates Mass spectrometry Tandem mass spectrometry Biochemistry 03 medical and health sciences Affinity chromatography Structural Biology Lectins Escherichia coli Animals Chemical Precipitation Molecular Biology Ammonium sulfate precipitation Chromatography 030102 biochemistry & molecular biology Molecular mass biology Chemistry Hemagglutination Lectin General Medicine Hydrogen-Ion Concentration Porifera Molecular Weight 030104 developmental biology Ammonium Sulfate Biofilms biology.protein Rabbits |
| Zdroj: | International journal of biological macromolecules. 99 |
| ISSN: | 1879-0003 |
| Popis: | A new lectin was isolated from the marine sponge Aplysina lactuca (ALL) by combining ammonium sulfate precipitation and affinity chromatography on guar gum matrix. ALL showed affinity for the disaccharides α-lactose, β-lactose and lactulose (Ka=12.5, 31.9 and 145.5M-1, respectively), as well as the glycoprotein porcine stomach mucin. Its hemagglutinating activity was stable in neutral acid pH values and temperatures below 60°C. ALL is a dimeric protein formed by two covalently linked polypeptide chains. The average molecular mass, as determined by Electrospray Ionization Mass Spectrometry (ESI-MS), was 31,810±2Da. ESI-MS data also indicated the presence of three cysteines involved in one intrachain and one interchain disulfide bond. The partial amino acid sequence of ALL was determined by tandem mass spectrometry. Eight tryptic peptides presented similarity with lectin I isolated from Axinella polypoides. Its secondary structure is predominantly β-sheet, as indicated by circular dichroism (CD) spectroscopy. ALL agglutinated gram-positive and gram-negative bacterial cells, and it were able to significantly reduce the biomass of the bacterial biofilm tested at dose- dependent effect. |
| Databáze: | OpenAIRE |
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