KGF Enhances Oral Epithelial Adhesion and Rete Peg Elongation via Integrins
| Autor: | Xuepeng Xiong, Y.F. Zhao, Hou-Fu Xia, Jian-Gang Ren, Jian Wang, Guoliang Sa, Zhikang Liu, Sangang He |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Integrins endocrine system Fibroblast Growth Factor 7 Blotting Western Integrin Cell Culture Techniques Real-Time Polymerase Chain Reaction Rats Sprague-Dawley Focal adhesion 03 medical and health sciences 0302 clinical medicine Cell-matrix adhesion Cell Adhesion Animals General Dentistry Matrigel biology Chemistry Hemidesmosome Mouth Mucosa Epithelial Cells Adhesion Rete pegs Rats Cell biology 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Oligopeptides |
| Zdroj: | Journal of Dental Research. 96:1546-1554 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034517720360 |
| Popis: | Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane. Keratinocyte growth factor (KGF) can promote both rete peg elongation in the skin and the expression of integrins in various cell types. Herein, we tested the effects of submucosal injection of KGF in the ventral surfaces of rat tongues on oral epithelial adhesion. The data confirmed that topical injection of KGF elevated the adhesive forces, elongated the rete pegs, and increased the abundance of integrins, KGF, and KGF receptor on the rat tongue ventral surface. However, HYD-1 (Lys-Ile-Lys-Met-Val-Ile-Ser-Trp-Lys-Gly), an integrin antagonist, inhibited the KGF-enhanced epithelial adhesion and rete peg elongation. Moreover, KGF promoted the expression of integrin subunits α6, β4, α3, and β1 in human immortalized oral epithelial cells in 2- and 3-dimensional culture systems. In vitro cell attachment assays demonstrated that HYD-1 inhibited the adhesion of human immortalized oral epithelial cells on Matrigel. Strikingly, the expression of integrins, KGF, and KGFR in human masticatory mucosae with longer rete pegs was more abundant than that in the lining mucosae with shorter rete pegs. In addition, rete peg lengths were positively correlated with the expression levels of integrins, KGF, and KGF receptor. These findings indicate that KGF strengthens oral epithelial adhesion and rete peg elongation via integrins. |
| Databáze: | OpenAIRE |
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