Microfluidic sperm selection yields higher sperm quality compared to conventional method in ICSI program: A pilot study

Autor:	Fatemeh Anbari, Fatemeh Dehghanpour, Mohammad Ali Khalili, Mojdeh Sabour, Ali Nabi, Abdul Munaf Sultan Ahamed, Esmat Mangoli
Rok vydání:	2021
Předmět:	Male 0301 basic medicine endocrine system Urology medicine.medical_treatment Microfluidics Pilot Projects DNA Fragmentation Biology Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Humans Sperm Injections Intracytoplasmic Sperm quality reproductive and urinary physiology Selection (genetic algorithm) 030219 obstetrics & reproductive medicine urogenital system Embryogenesis Spermatozoa Sperm Chromatin 030104 developmental biology Reproductive Medicine Female
Zdroj:	Systems Biology in Reproductive Medicine. 67:137-143
ISSN:	1939-6376 1939-6368
DOI:	10.1080/19396368.2020.1837994
Popis:	An appropriate preparation technique, should be capable of isolating highquality spermatozoa for intracytoplasmic sperm injection (ICSI). The aim was to assess sperm quality parameters, DNA integrity, embryo development, and clinical outcomes using a practical and accessible Microfluidic Sperm Sorting (MSS) technique. A total of 95 ICSI cases performed using sperm samples were prepared with our MSS (group 1) or by Direct Swim Up (DSU; control) method (group 2). Both sperm quality parameters and sperm DNA fragmentation (SDF) were compared between the groups. DNA fragmentation was assessed using Sperm Chromatin Dispersion (SCD) test and fine morphology was assessed using Motile Sperm Organelle Morphology Examination (MSOME). Embryo development and clinical outcomes were compared between the groups. In the MSS group, progressive motility and the fraction of Class I sperm morphology sperm were significantly higher compared to DSU group (P 0.01 and P 0.001, respectively). Moreover, the rates of DNA fragmentation and immotile spermatozoa were significantly lower in MSS when compared to DSU group (P 0.001). Also, higher rates of high-quality embryo formation (P 0.001), implantation (P = 0.04) and pregnancy (P = 0.05) were achieved in the MSS compared to DSU groups. The MSS technique proved to be a noninvasive, disposable, easy to use, and inexpensive method for separation of high-quality spermatozoa. Both laboratory parameters and clinical outcomes were improved with application of MSS for neat sperm collection in ICSI.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bbc21029b8182d32d73a2169467d369 https://doi.org/10.1080/19396368.2020.1837994 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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