S-phase inhibitors induce vimentin expression in human promonocytic U-937 cells
| Autor: | P. Aller, Carmelo Bernabeu, Felicísima Mata, C. Rius, Carlos Cabañas |
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| Rok vydání: | 1989 |
| Předmět: |
Cellular differentiation
Biophysics Antigens Differentiation Myelomonocytic Vimentin Biology Biochemistry Flow cytometry Antigen Structural Biology Gene expression Tumor Cells Cultured Genetics medicine Humans Hydroxyurea RNA Messenger Northern blot Interphase Cytosine arabinoside Molecular Biology Cytoskeleton medicine.diagnostic_test Cell Cycle Cytarabine Cell Differentiation Cell Biology Cell cycle Blotting Northern medicine.disease Molecular biology Leukemia Gene Expression Regulation U-937 cell Differentiation biology.protein Tetradecanoylphorbol Acetate |
| Zdroj: | FEBS Letters. 259:171-174 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(89)81521-2 |
| Popis: | The administration of hydroxyurea (3 x 10(-4) M) and cytosine arabinoside (10(-7) M) greatly induces the expression of the vimentin gene in human promonocytic leukemia U-937 cells. The induction takes place at both the mRNA and protein levels, as demonstrated by Northern blot, immunoblot and immunofluorescence assays. On the contrary, the drugs inhibit the expression of c-myc and ornithine decarboxylase, and do not modify significantly the expression of beta-actin. Since hydroxyurea and cytosine arabinoside trigger the phenotypic differentiation of U-937 cells, as demonstrated by the induction of the differentiation-specific CD11b and CD11c antigens, it is concluded that vimentin expression might be implicated in the maturation of these cells. |
| Databáze: | OpenAIRE |
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