Proof of concept study of mass cytometry in septic shock patients reveals novel immune alterations
| Autor: | Sophie Debord, Thibault Andrieu, Christophe Malcus, Fabienne Venet, Guillaume Monneret, Françoise Poitevin-Later, Frédérique Bayle, Morgane Gossez, Thomas Rimmelé |
|---|---|
| Přispěvatelé: | Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques / Pathophysiology of Injury-induced Immunosuppression (PI3), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Hospices Civils de Lyon, Immunology Laboratory, Edouard Herriot Hospital, Lyon, France, Anesthesia and Critical Care Medicine Department, Hospices Civils de Lyon, Edouard Herriot Hospital, 69437, Lyon, France., SFR Biosciences, École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine T-Lymphocytes medicine.medical_treatment [SDV]Life Sciences [q-bio] lcsh:Medicine Lymphocyte Activation Proof of Concept Study B7-H1 Antigen Monocytes Article Sepsis 03 medical and health sciences 0302 clinical medicine Immune system Plasma cell differentiation medicine Humans Mass cytometry lcsh:Science B cell Multidisciplinary business.industry Septic shock Monocyte lcsh:R HLA-DR Antigens Immunotherapy Middle Aged Flow Cytometry medicine.disease Shock Septic 030104 developmental biology medicine.anatomical_structure Case-Control Studies Immunology Female lcsh:Q Single-Cell Analysis business Biomarkers 030215 immunology |
| Zdroj: | Scientific Reports Scientific Reports, 2018, 8 (1), pp.17296. ⟨10.1038/s41598-018-35932-0⟩ Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-35932-0⟩ |
| Popis: | Innovative single cell technologies such as mass cytometry (CyTOF) widen possibilities to deeply improve characterisation of immune alterations mechanisms in human diseases. So far, CyTOF has not been used in sepsis – a condition characterized by complex immune disorders. Here, we evaluated feasibility of CyTOF analysis in patients with septic shock. We designed a mass cytometry panel of 25 extracellular markers to study mononuclear cells from 5 septic shock patients and 5 healthy donors. We explored single-cell data with global and specific unsupervised approaches such as heatmaps, SPADE and viSNE. We first validated relevance of our CyTOF results by highlighting established immune hallmarks of sepsis, such as decreased monocyte HLA-DR expression and increased expressions of PD1 and PD-L1 on CD4 T cells and monocytes. We then showed that CyTOF analysis reveals novel aspects of sepsis-induced immune alterations, e.g. B cell shift towards plasma cell differentiation and uniform response of several monocyte markers defining an immune signature in septic patients. This proof of concept study demonstrates CyTOF suitability to analyse immune features of septic patients. Mass cytometry could thus represent a powerful tool to identify novel pathophysiological mechanisms and therapeutic targets for immunotherapy in septic shock patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|