A protein interaction map of the LSU processome
| Autor: | Nicholas G. Vincent, J. Michael Charette, Susan J. Baserga, Kathleen L. McCann |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Ribosomal Proteins
Genetics Saccharomyces cerevisiae Proteins Systems biology Two-hybrid screening Reproducibility of Results Ribosome biogenesis Saccharomyces cerevisiae Computational biology Biology Ribosome Interactome Ribosomal protein Two-Hybrid System Techniques Large ribosomal subunit Protein Interaction Maps Resource/Methodology Biogenesis Developmental Biology |
| Zdroj: | Genes & Development. 29:862-875 |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | Maturation of the large ribosomal subunit (LSU) in eukaryotes is a complex and highly coordinated process that requires the concerted action of a large, dynamic, ribonucleoprotein complex, the LSU processome. While we know that >80 ribosome biogenesis factors are required throughout the course of LSU assembly, little is known about how these factors interact with each other within the LSU processome. To interrogate its organization and architecture, we took a systems biology approach and performed a semi-high-throughput, array-based, directed yeast two-hybrid assay. Assaying 4800 protein–protein interactions, we identified 232 high-confidence, binary-interacting protein pairs, representing a fourfold increase from current knowledge. The resulting LSU processome interactome map has enhanced our understanding of the organization and function of the biogenesis factors within the LSU processome, revealing both novel and previously identified subcomplexes and hub proteins, including Nop4. |
| Databáze: | OpenAIRE |
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