A microfluidic system for high-speed reproducible DNA sizing and quantitation
| Autor: | Karen Hahnenberger, Rob Nagle, Odilo Mueller, Robert S. Dubrow, Monika Dittmann, Herman Yee, Diane D. Ilsley |
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| Rok vydání: | 2000 |
| Předmět: |
Electrophoresis
Chromatography Materials science Clinical Biochemistry Microfluidics Analytical chemistry DNA Biochemistry Analytical Chemistry law.invention Matrix (chemical analysis) Diffusion chemistry.chemical_compound Plasmid chemistry law Animals Humans Restriction fragment length polymorphism Polymerase chain reaction Microfabrication |
| Zdroj: | Electrophoresis. 21(1) |
| ISSN: | 0173-0835 |
| Popis: | Microfabrication technology was used to develop a system consisting of disposable glass chips containing etched channels, reagents including polymer matrix and size standards, computer-controlled instrumentation for performing electrophoretic separations and fluorescence detection of double-stranded DNA, and software for automated data analysis. System performance was validated for separation and quantitation reproducibility using samples varying in amount and size of DNA fragments, buffer composition, and salt concentrations. Several applications of the microfluidic system for DNA analysis have been demonstrated, such as of polymerase chain reaction (PCR) products, sizing of plasmid digests, and detection of point mutations by restriction fragment length polymorphism (RFLP) mapping. |
| Databáze: | OpenAIRE |
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