The effect of the mitochondrial permeability transition pore on apoptosis in Eimeria tenella host cells

Autor: Sha-sha Yang, Xiao-zhen Cui, Rui Bai, Shan Li, Zhi-yong Xu, Qiang-hua Lv, Wen-long Zhao, Ming-xue Zheng, Rui-li Liu, Yan Zhang
Rok vydání: 2016
Předmět:
0301 basic medicine
Necrosis
animal diseases
Apoptosis
Chick Embryo
Mitochondrial Membrane Transport Proteins
Eimeria
Flow cytometry
Host-Parasite Interactions
Avian Proteins
03 medical and health sciences
chemistry.chemical_compound
Cyclosporin a
parasitic diseases
medicine
Animals
Cecum
Cells
Cultured
Poultry Diseases
Membrane Potential
Mitochondrial
medicine.diagnostic_test
biology
Coccidiosis
Mitochondrial Permeability Transition Pore
MPTP
0402 animal and dairy science
Epithelial Cells
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Flow Cytometry
040201 dairy & animal science
Molecular biology
In vitro
Cell biology
Specific Pathogen-Free Organisms
030104 developmental biology
chemistry
Mitochondrial permeability transition pore
Cyclosporine
Animal Science and Zoology
medicine.symptom
Chickens
Eimeria tenella
Zdroj: Poultry science. 95(10)
ISSN: 1525-3171
Popis: Although the mitochondrial permeability transition pore (MPTP) is associated with cellular apoptosis and necrosis, its effect in host response to Eimeria infections is not well understood. In an effort to better understand the effect of MPTP on apoptosis in Eimeria tenella host cells, an MPTP inhibitor (cyclosporin A) was used to inhibit MPTP opening in vitro. Cecal epithelial cells from chick embryos, which were either treated or non-treated with cyclosporin A, were used as Eimeria tenella host cells. In addition, primary chick embryo cecum epithelial cell culture techniques and flow cytometry were used to detect the dynamic changes in MPTP opening, mitochondrial transmembrane potential, and cell apoptosis rate of Eimeria tenella host cells. Compared with the control group, cytometric techniques showed that untreated host cells exhibited a significantly higher (P < 0.01) degree of MPTP opening but lower (P < 0.01 or P < 0.05) mitochondrial transmembrane potential. Moreover, untreated group cells had less apoptosis (P < 0.01) at 4 h and more apoptosis (P < 0.05 or P < 0.01) at 24 to 120 h as compared with control group cells. After the application of cyclosporin A, the degree of MPTP opening in the treated group was significantly lower (P < 0.01) at 4 to 120 h compared to the untreated group, whereas the treated group had higher (P < 0.05 or P < 0.01) mitochondrial transmembrane potentials at 24 to 120 h. Flow cytometry assays also showed that there was less (P < 0.05 or P < 0.01) apoptosis after 24 h in the treated group than in the untreated group. Taken together, these observations indicate that MPTP is a key node that plays a predominant role in the mitochondrial apoptosis pathway in the host cell induced by Eimeria tenella.
Databáze: OpenAIRE
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