Near-infrared branding efficiently correlates light and electron microscopy
| Autor: | Ivana Nikić, Thomas Misgeld, Stephanie Potz, Mary Brinkoetter, Martin Kerschensteiner, Sharmon M. Knecht, Derron L. Bishop |
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| Rok vydání: | 2011 |
| Předmět: |
Tissue Fixation
Materials science Infrared Rays Infrared Electrons Biochemistry Fluorescence law.invention Mice Optics Fiducial Markers law Microscopy Animals Molecular Biology Cerebral Cortex business.industry Lasers Macrophages Scanning confocal electron microscopy Cell Biology Laser Dark field microscopy Microscopy Electron Kidney Tubules Lymph Nodes Electron microscope business Fiducial marker Biotechnology |
| Zdroj: | Nature Methods. 8:568-570 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.1622 |
| Popis: | The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy. |
| Databáze: | OpenAIRE |
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