Synthesis, characterization and antimicrobial activity of conjugates based on fluoroquinolon-type antibiotics and gelatin
Autor: | Cristina Giraldi, Francesco Puoci, Maria Vittoria Mauro, Nevio Picci, Orazio Vittorio, Francesca Iemma, Giuseppe Cirillo, Paolina Cavalcanti, Umile Gianfranco Spizzirri |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Materials science
food.ingredient Biocompatibility Klebsiella pneumoniae medicine.drug_class Antibiotics Biomedical Engineering Biophysics Bioengineering Biocompatible Materials Microbial Sensitivity Tests medicine.disease_cause Gelatin Biomaterials Minimum inhibitory concentration food Materials Testing medicine Escherichia coli Humans Cells Cultured biology Molecular Structure Biological activity biology.organism_classification Antimicrobial Hematopoietic Stem Cells Anti-Bacterial Agents Biochemistry Fluoroquinolones |
Zdroj: | Journal of materials science. Materials in medicine (Dordr., Online) 25 (2014): 67–77. doi:10.1007/s10856-013-5053-8 info:cnr-pdr/source/autori:Cirillo G. [ 1,2 ] ; Mauro M.V.[ 3 ] ; Spizzirri U.G. [ 1 ]; Cavalcanti P. [ 3 ]; Puoci F.[ 1 ] ; Giraldi C.[ 3 ] ; Vittorio O.[ 4,5,6,7 ] ; Picci N.[ 1 ]; Iemma F.[ 1 ]/titolo:Synthesis, characterization and antimicrobial activity of conjugates based on fluoroquinolon-type antibiotics and gelatin/doi:10.1007%2Fs10856-013-5053-8/rivista:Journal of materials science. Materials in medicine (Dordr., Online)/anno:2014/pagina_da:67/pagina_a:77/intervallo_pagine:67–77/volume:25 |
Popis: | Different fluoroquinolon-type antibiotics were conjugated to gelatin with the aim to synthesize biomacromolecules with antimicrobial properties. The covalent linkage of the antibiotic was performed by a radical process involving the residues in the side chains of gelatin able to undergo oxidative modifications. The conjugation of antibiotic moieties onto the protein structure was confirmed by FT-IR, UV-Vis, fluorescence, and calorimetric analyses. Biocompatibility tests were performed on human bone marrow mesenchymal stromal cells and the antibacterial properties of bioactive polymers were investigated by appropriate tests against Klebsiella pneumoniae and Escherichia coli. With regard to the tests conducted in the presence of E. coli, a minimum inhibitory concentration (MIC) ranging from 0.05 to 0.40 mu g mL(-1) was recorded, while in the presence of K. pneumoniae this concentration varies from 0.10 to 1.60 mu g mL(-1). In all the conjugates, the drug moieties retain their biological activity and the MIC values are lower than the resistance parameters of fluoroquinolon-type antibiotics versus Enterobacteriacae. The collected data suggest a broad range of applications, from biomedical to pharmaceutical and food science for all conjugates. |
Databáze: | OpenAIRE |
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