Two dimensional difference gel electrophoresis analysis of cerebrospinal fluid in tuberculous meningitis patients
| Autor: | Jitender Kataria, Manjari Tripathi, Lokesh A. Rukmangadachar, Gururao Hariprasad, O. Jithesh, Alagiri Srinivasan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Fungal meningitis Pathology medicine.medical_specialty Difference gel electrophoresis Biophysics Meningitis Cryptococcal urologic and male genital diseases Biochemistry Mass Spectrometry Tuberculous meningitis Two-Dimensional Difference Gel Electrophoresis Mycobacterium tuberculosis Cerebrospinal fluid Western blot Glial Fibrillary Acidic Protein medicine Humans Arachidonate 5-Lipoxygenase biology Glial fibrillary acidic protein medicine.diagnostic_test biology.organism_classification medicine.disease Molecular biology Tuberculosis Meningeal biology.protein Female Biomarkers |
| Zdroj: | Journal of Proteomics. 74:2194-2203 |
| ISSN: | 1874-3919 |
| Popis: | Tuberculous meningitis (TBM) is a serious complication of tuberculosis that affects the central nervous system. Present methods to diagnose TBM are not suitable for early diagnosis. Molecular markers and sensitive methods to identify them in the early stage of infection of TBM are critically needed for efficient management. We have done the proteomic analysis of TBM cerebrospinal fluid (n=20) with 2-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. We identified 11 human proteins and 8 mycobacterial proteins with changed expression levels in comparison to controls. Arachidonate 5-lipoxygenase and glial fibrillary acidic protein, two of the identified proteins, were validated with western blot technique on a larger set of disease and control samples (n=40). These two proteins were also analyzed in fungal meningitis samples. We suggest that arachidonate 5-lipoxygenase can be considered for validation as a potential marker for diagnosis of TBM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect