| Autor: |
Taku Mizutani, Ryotaro Hara, Michiki Takeuchi, Kazuo Yamagishi, Yoshinori Hirao, Kenichi Mori, Makoto Hibi, Makoto Ueda, Jun Ogawa |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Bioscience, biotechnology, and biochemistry. 86(6) |
| ISSN: |
1347-6947 |
| Popis: |
S-Allyl-l-cysteine (SAC) has received much interest due to its beneficial effects on human health. To satisfy the increasing demand for SAC, this study aims to develop a valuable culturing method for microbial screening synthesizing SAC from readily available materials. Although tryptophan synthase is a promising enzyme for SAC synthesis, its expression in microorganisms is strictly regulated by environmental l-tryptophan. Thus, we constructed a semisynthetic medium lacking l-tryptophan using casamino acids. This medium successfully enhanced the SAC-synthesizing activity of Lactococcus lactis ssp. cremoris NBRC 100676. In addition, microorganisms with high SAC-synthesizing activity were screened by the same medium. Food-related Klebsiella pneumoniae K-15 and Pantoea agglomerans P-3 were found to have a significantly increased SAC-synthesizing activity. The SAC-producing process established in this study is shorter in duration than the conventional garlic aging method. Furthermore, this study proposes a promising alternative strategy for producing food-grade SAC by microorganisms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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