In situ immunodetection of activated caspase-3 in apoptotic neurons in the developing nervous system
| Autor: | Anu Srinivasan, Kevin J. Tomaselli, Kevin A. Roth, Lawrence C. Fritz, Kenneth S. Shindler, Robert O. Sayers, Angela M. Wong |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Nervous system
Male Programmed cell death Antimetabolites Antineoplastic bcl-X Protein Fluorescent Antibody Technique Gene Expression Caspase 3 Apoptosis Cross Reactions Cysteine Proteinase Inhibitors Jurkat Cells Mice Bcl-2-associated X protein In vivo Antibody Specificity Coumarins Zymogen Proto-Oncogene Proteins medicine Animals Humans Molecular Biology bcl-2-Associated X Protein Antiserum Cerebral Cortex Mice Knockout Neurons Enzyme Precursors biology Cytarabine Brain Cell Biology Molecular biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Caspases biology.protein Female Rabbits Oligopeptides |
| Zdroj: | Cell death and differentiation. 5(12) |
| ISSN: | 1350-9047 |
| Popis: | Activation of caspase-3 requires proteolytic processing of the inactive zymogen into p18 and p12 subunits. We generated a rabbit polyclonal antiserum, CM1, which recognizes the p18 subunit of cleaved caspase-3 but not the zymogen. CM1 demonstrated an apparent specificity for activated caspase-3 by specifically immunolabelling only apoptotic but not necrotic cortical neurons in vitro. In the embryonic mouse nervous system, CM1 immunoreactivity was detected in neurons undergoing programmed cell death and was markedly increased in Bcl-xL-deficient embryos and decreased in Bax-deficient embryos. CM1 immunoreactivity was absent in the nervous system of caspase-3-deficient mouse embryos and in neurons cultured from caspase-3-deficient mice. Along with neuronal somata, extensive neuritic staining was seen in apoptotic neurons. These studies indicate that caspase-3 is activated during apoptosis in the developing nervous system in vivo and that CM1 is a useful reagent for its in situ detection. |
| Databáze: | OpenAIRE |
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