Determination of disulfide bridges in natural and recombinant insect defensin A
| Autor: | Jules A. Hoffmann, F. Bitsch, Jean-Luc Dimarcq, Elisabeth Keppi, Jean-Marc Reichhart, Pierre Lepage, Carolyn Roitsch, Alain Van Dorsselaer, D. Roecklin |
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| Přispěvatelé: | Réponse immunitaire et developpement chez les insectes (RIDI - UPR 9002), Université de Strasbourg (UNISTRA)-Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Modèles Insectes de l'Immunité Innée (M3I), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), univOAK, Archive ouverte |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
[SDV.IMM] Life Sciences [q-bio]/Immunology
Molecular Sequence Data Saccharomyces cerevisiae Peptide Biology Mass spectrometry Biochemistry Mass Spectrometry law.invention Defensins Thermolysin law Hemolymph Animals Amino Acid Sequence Disulfides Defensin chemistry.chemical_classification Chromatography Edman degradation integumentary system Diptera fungi Protein primary structure Blood Proteins biology.organism_classification Recombinant Proteins chemistry Recombinant DNA [SDV.IMM]Life Sciences [q-bio]/Immunology |
| Zdroj: | European Journal of Biochemistry European Journal of Biochemistry, Wiley, 1991, 196 (3), pp.735-742 |
| ISSN: | 0014-2956 1432-1327 |
| Popis: | The primary-structure comparison of natural insect defensin A from Phormia terranovae and recombinant insect defensin A from Saccharomyces cerevisiae has been accomplished using a combination of Edman degradation and liquid secondary ion mass spectrometry. The natural and recombinant proteins have the same primary structure with identical disulfide-bond designations (formula; see text) as determined from the peptides obtained after thermolysin digestion. The combined use of Edman degradation and mass spectometry allowed the disulfide-bridge structure to be determined with a total of only 40 micrograms (9.9 nmol) natural peptide. Mass spectrometry provides a rapid means of disulfide-bridge verification, requiring not more than 20 micrograms recombinant insect defensin A, which is compatible with use in batch analysis. |
| Databáze: | OpenAIRE |
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