Neuropeptide W Exhibits Preventive and Therapeutic Effects on Acetic Acid-Induced Colitis via Modulation of the Cyclooxygenase Enzyme System
| Autor: | Sevil Arabacı Tamer, Selin Akbulut, Ömer Erdoğan, Özge Çevik, Feriha Ercan, Berrak Ç. Yeğen |
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| Přispěvatelé: | ARABACI TAMER S., Akbulut S., Erdogan O., Cevik O., ERCAN F., YEGEN B. |
| Rok vydání: | 2023 |
| Předmět: |
Internal Diseases
EXPRESSION STRESS Physiology PATHOGENESIS Gastroenterology and Hepatology Sağlık Bilimleri İç Hastalıkları Clinical Medicine (MED) RATS Gastroenteroloji-(Hepatoloji) Health Sciences Neuropeptide W Klinik Tıp (MED) NITRIC-OXIDE SYNTHASE Oxidative injury Inflammation Internal Medicine Sciences Klinik Tıp Hepatology BLOOD-FLOW GASTROENTEROLOGY & HEPATOLOGY Gastroenterology Dahili Tıp Bilimleri CLINICAL MEDICINE GASTROENTEROLOJİ VE HEPATOLOJİ Cyclooxygenases CROHNS-DISEASE Tıp Hepatoloji Gastroenteroloji Ulcerative colitis ULCERATIVE-COLITIS CELLS Medicine INFLAMMATORY-BOWEL-DISEASE |
| Zdroj: | Digestive Diseases and Sciences. 68:2441-2453 |
| ISSN: | 1573-2568 0163-2116 |
| Popis: | Background The novel peptide neuropeptide W (NPW) was originally shown to function in the control of feeding behavior and energy homeostasis. The aim of this study was to elucidate the putative preventive and therapeutic effects of NPW on colitis-associated oxidative injury and the underlying mechanisms for its action.Methods Sprague-Dawley rats in the acute colitis groups received NPW (0.5, 1 or 5 mu g/kg/day) injections prior to induction of colitis with acetic acid, while the chronic colitis groups were treated after the induction of colitis. In both acute and chronic colitis (CC) groups, treatments were continued for 5 days and the rats were decapitated at the 24th hour of the last injections and colon tissues were collected for assessments.Results NPW pretreatment given for 5 days before colitis induction, as well as treating rats with NPW during the 5-day course of CC, abolished colonic lipid peroxidation. NPW treatment prevented colitis-induced reduction in blood flow, diminished neutrophil infiltration, and pro-inflammatory cytokine responses. NPW pretreatment only at the higher dose reduced colonic edema and microscopic score and preserved colonic glutathione stores. Elevations in cyclooxygenase (COX) enzyme activity and COX-1 protein level during the acute phase of colitis as well as reduction in COX-2 were all reversed with NPW pretreatment. In contrast, NPW treatment was effective in reducing the elevated COX-2 concentration during the chronic phase.Conclusions NPW alleviates acetic acid-induced oxidative colonic injury in rats through the upregulation of colonic blood flow as well as the inhibition of COX-2 protein expression and pro-inflammatory cytokine production. |
| Databáze: | OpenAIRE |
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