Lon domain-containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts
| Autor: | Soo Youn Kim, Minsoo Noh, Jin Seok Shin, Jeong Sheop Shin, Won Mi So, Kyoung Shin Yoo |
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| Rok vydání: | 2019 |
| Předmět: |
Biophysics
Arabidopsis Flowers Biochemistry Lignin 03 medical and health sciences chemistry.chemical_compound Thioredoxins Structural Biology Gene Expression Regulation Plant Genetics Molecular Biology Gene 030304 developmental biology 0303 health sciences Gene knockdown biology Chemistry Arabidopsis Proteins 030302 biochemistry & molecular biology food and beverages Cell Biology Hydrogen Peroxide biology.organism_classification Cell biology Chloroplast Thioredoxin Function (biology) |
| Zdroj: | FEBS lettersReferences. 594(6) |
| ISSN: | 1873-3468 |
| Popis: | Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain-containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx-y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS-responsive marker genes than the wild-type plant. Taken together, we suggest that LCP1 directly regulates Trx-y2 and controls H2 O2 levels and, thereby, regulates lignin polymerization in the anther endothecium. |
| Databáze: | OpenAIRE |
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