Microfluidic device for label-free quantitation and distinction of bladder cancer cells from the blood cells using micro machined silicon based electrical approach; suitable in urinalysis assays
| Autor: | Mohammad Abdolahad, Somayeh Zanganeh, Seied Ali Hosseini, Elaheh Akbarnejad, Fatemeh Salehi |
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| Rok vydání: | 2017 |
| Předmět: |
Silicon
Urinalysis Clinical Biochemistry Microfluidics Pharmaceutical Science Nanotechnology 02 engineering and technology Urine 01 natural sciences Capacitance Analytical Chemistry Lab-On-A-Chip Devices Drug Discovery Leukocytes medicine Humans Spectroscopy Detection limit Blood Cells Bladder cancer medicine.diagnostic_test Chemistry 010401 analytical chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Label-free quantification Urinary Bladder Neoplasms Cancer cell 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 134:36-42 |
| ISSN: | 0731-7085 |
| Popis: | This paper introduces an integrated microfluidic chip as a promising tool to measure the concentration of bladder cancer cells (BCC) in urine samples. Silicon microchannels were used as trapping gates for both floated BCC and leukocytes which are found in the urine of patients. By the assistance of the gold electrodes patterned at the bottom of the micro gates, the capacitance of captured cancerous and blood cells were measured. Different membrane capacitance between BCC and leukocyte was the indicative signal for diagnosing the nature of captured cells in a urine like solution. The concentration range of the target that could be detected was about 10 BCCs per one chip. Such response has been achieved without applying any biochemical or florescent markers. Thus, it could be a simple and cheap approach to support cytological and immune-fluorescent assays. The limit of detection was approximately 1 cancerous cell/11 leukocytes in 1ml of the urine like solution. The entire measurement time was less than an hour. Consequently, this electrical microfluidic device promises significant potential in urinalysis. |
| Databáze: | OpenAIRE |
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