Oxidative stress induced by self-adhesive resin cements affects gene expression, cellular proliferation and mineralization potential of the MDPC-23 odontoblast-like cells
Autor: | Marcela Maciel Palacio Alvarez, Ivarne L.S. Tersariol, Silvana Coelho de Arruda Barbosa, Fabio D. Nascimento, Mackeler Ramos Polassi, Rafael Guzella de Carvalho, Paulo Henrique Perlatti D'Alpino |
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Rok vydání: | 2018 |
Předmět: |
Materials science
NF-E2-Related Factor 2 Dental Cements 02 engineering and technology medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Gene expression Materials Testing medicine Cytotoxic T cell Gelatinase Animals General Materials Science General Dentistry Cell Proliferation Kelch-Like ECH-Associated Protein 1 Odontoblasts Cell growth Dental Bonding 030206 dentistry 021001 nanoscience & nanotechnology Cell biology Rats Resin Cements Oxidative Stress Odontoblast Mechanics of Materials Dentin Tumor necrosis factor alpha Signal transduction 0210 nano-technology Oxidative stress |
Zdroj: | Dental materials : official publication of the Academy of Dental Materials. 35(4) |
ISSN: | 1879-0097 |
Popis: | Objective Clinical issues have been raised about problems related to cytotoxic effects caused when applying self-adhesive cement. It was hypothesized that byproducts eluted from self-adhesive cements modulate oxidative stress response, the gene expression of signaling pathways of inflammatory process/transcriptional activators, and the expression and activity of interstitial collagenases, and modify the phenotypic characteristics of cellular proliferation and mineral deposition in odontoblastic-like cells. Methods Cements (MaxCem Elite [MAX] and RelyX U200 [U200)]) were mixed, dispensed into moulds, and photoactivated according to the manufacturers’ instructions. Immortalized rat odontoblast-like cells (MDPC-23) were cultured and exposed to polymerized specimens of cements for 4 h. Reactive oxidative specimen production and quantification of gene expression were evaluated. Cell proliferation assay and alizarin red staining were also performed to evaluate the disturbance induced by the cements on cellular proliferation and mineralization. Results Despite their cytotoxic effects, both self-adhesive cements influenced the metabolism in the odontoblast cells on different scales. MAX induced significantly higher oxidative stress in odontoblast cells than U200. Gene expression varied as a function of exposure to self-adhesive cements; MAX induced the expression of pro-inflammatory cytokines such as TNF-α, whereas U200 downregulated, virtually depleted TNF-α expression, also inducing overexpression of the transcriptional factor Runx2. Overexpression of heme oxygenase-1 (HO-1) and thioredoxin reductase 1 (TRXR1) occurred after exposure to both cements, antioxidant genes that are downstream of Keap1-Nrf2-ARE system. MAX significantly induced the overexpression of collagenase MMP-1, and U200 induced the expression of gelatinase MMP-2. MAX significantly inhibited cell proliferation whereas U200 significantly activated cell proliferation. Alizarin red staining revealed significantly decreased mineral deposition especially when exposed to MAX. Significance These results support the hypothesis that byproducts of different self-adhesive cements play important roles in the highly orchestrated process which ultimately affect the cellular proliferation and the mineral deposition in odontoblastic-like cells, possibly delaying the reparative dentin formation after cementation of indirect restorations, especially on recently exposed dentin preparations. |
Databáze: | OpenAIRE |
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