Aquaspirillum soli sp. nov., isolated from a soil sample
| Autor: | Huan Trinh, Gabriela Moya, KyungHwa Won, Zheng-Fei Yan, Qi-Jun Wang, Jung-Eun Yang, Tae-Hoo Yi, MooChang Kook |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial China Ubiquinone Microbiology 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Botany Microaerophile Gene Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology Phosphatidylglycerol Base Composition biology Phylogenetic tree Strain (chemistry) Fatty Acids Betaproteobacteria Nucleic Acid Hybridization General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology chemistry Bacteria Aquaspirillum serpens |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(5) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-negative, smooth, opaque, white-pigmented, helical-shaped, catalase- and oxidase-positive bacterium that was motile by means of bipolar tufts of flagella and grew under microaerophilic conditions, was isolated from a soil sample of a reed pond in Shangqiu, Henan province, PR China. The strain, designated THG-SQE6T, grows well at 25–42 °C, pH 6.5–7.5 and in the presence of 0–1 % (w/v) NaCl. hylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-SQE6T was most closely related to Aquaspirillum serpens IAM 13944T (97.23 % 16S rRNA gene sequence similarity). The DNA G+C content of strain THG-SQE6T was 53.10 mol%. In DNA–DNA hybridization experiments, the DNA–DNA relatedness between strain THG-SQE6T and its closest phylogenetic neighbour was below 62.6 %. The predominant isoprenoid quinone detected in strain THG-SQE6T was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. The major fatty acids were identified as C12 : 0 3-OH, C16 : 0, C18 : 0, summed feature 3 and summed feature 8. These data support the affiliation of strain THG-SQE6T to the genus Aquaspirillum . Based on findings from the phenotypic, genotypic and phylogenetic characterization of strain THG-SQE6T, a novel species of the genus Aquaspirillum named Aquaspirillum soli sp. nov. is proposed. The type strain is THG-SQE6T (=KACC 18846T=CCTCC AB 2016081T). |
| Databáze: | OpenAIRE |
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