SELDI-TOF MS Analysis of Hepatocellular Carcinoma in an Australian Cohort
| Autor: | Aiqun Xue, Anthony J. Gill, Ross C. Smith, Thomas J. Hugh, Christopher B. Nahm, Steven Schlichtemeier |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Oncology
Male Proteomics Prognostic factor medicine.medical_specialty Multivariate analysis Carcinoma Hepatocellular Disease-Free Survival Resection 03 medical and health sciences 0302 clinical medicine Internal medicine SELDI-TOF-MS medicine Biomarkers Tumor Hepatectomy Humans Neoplasm Invasiveness Prospective Studies Aged business.industry Liver Neoplasms Australia Middle Aged medicine.disease Prognosis Survival Analysis Liver 030220 oncology & carcinogenesis Hepatocellular carcinoma Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cohort Microvessels 030211 gastroenterology & hepatology Surgery Female business Area under the roc curve |
| Zdroj: | The Journal of surgical research. 238 |
| ISSN: | 1095-8673 |
| Popis: | Hepatocellular carcinoma (HCC) is a common cause of cancer death worldwide. Resection offers the best chance of long-term survival, but a consistent adverse prognostic factor is the presence of microvascular invasion (MVI). In this study, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), a high throughput method of analyzing complex samples, was used to explore differentially expressed proteins between HCC and adjacent nontumour liver tissue (ANLT). These findings were correlated with clinical outcomes.From 2002 to 2011, tumor and ANLT were collected from patients who underwent liver resection and these samples were later prepared for SELDI-TOF MS. Output data were then used to identify proteins capable of discriminating HCC from ANLT. Proteins from the multivariate analysis were then analyzed to determine prognostic factors and the m/z ratios of these proteins were entered into the ExPASy database to infer potential candidates.During the study period, 30 patients had SELDI-TOF MS performed on their HCC and ANLT samples. On multivariate analysis, a panel of four proteins-m/z 5840, m/z 8921, m/z 9961, and m/z 25,872-discriminated HCC from ANLT with an area under the ROC curve of 0.954 (P 0.001). On prognostic factor assessment, decreased m/z 9961 was significantly associated with the presence of MVI (P = 0.025) and shorter disease-free survival (P = 0.045) in our patients. A potential candidate for this protein was coxsackievirus and adenovirus receptor, isoform 3 (CAR 3/7), which helps maintain tight junction integrity.Using SELDI TOF-MS, we identified a panel of four proteins with excellent discriminative capacity between HCC and ANLT. Of these, m/z 9961 was the only protein significantly associated with a known poor prognostic factor (presence of MVI) and survival (shorter disease-free survival). While loss of CAR 3/7 could lead to MVI, further research is warranted to validate the identity of protein m/z 9961. |
| Databáze: | OpenAIRE |
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