Integrated Graphene Oxide with Noble Metal Nanoparticles to Develop High-Sensitivity Fiber Optic Particle Plasmon Resonance (FOPPR) Biosensor for Biomolecules Determination
| Autor: | Lai-Kwan Chau, Chien-Hsing Chen, Chin-Wei Wu, Chien-Tsung Wang, Chang-Yue Chiang |
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| Rok vydání: | 2021 |
| Předmět: |
fiber optic
Analyte Materials science IgG Calibration curve General Chemical Engineering Analytical chemistry 02 engineering and technology biosensor 01 natural sciences Article law.invention lcsh:Chemistry law anti-IgG General Materials Science Surface plasmon resonance Detection limit Graphene 010401 analytical chemistry particle plasmon resonance 021001 nanoscience & nanotechnology 0104 chemical sciences lcsh:QD1-999 Linear range Colloidal gold gold nanoparticles graphene oxide 0210 nano-technology Biosensor |
| Zdroj: | Nanomaterials Nanomaterials, Vol 11, Iss 635, p 635 (2021) Volume 11 Issue 3 |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11030635 |
| Popis: | In this research, a direct, simple and ultrasensitive fiber optic particle plasmon resonance (FOPPR) biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide (AuNP/GO) composite as signal amplification element. To obtain the best analytical performance of the sensor, experimental parameters including the surface concentration of GO on the AuNPs, formation time of the GO, the concentration of the anti-IgG and incubation time of anti-IgG were optimized. The calibration plots displayed a good linear relationship between the sensor response (ΔI/I0) and the logarithm of the analyte concentrations over a linear range from 1.0 × 10−10 to 1.0 × 10−6 g/mL of IgG under the optimum conditions. A limit of detection (LOD) of 0.038 ng/mL for IgG was calculated from the standard calibration curve. The plot has a linear relationship (correlation coefficient, R = 0.9990). The analytical performance of present work’s biosensor was better than that of our previously reported mixed self-assembled monolayer of 11-mercaptoundecanoic acid/6-mercapto-1-hexanol (MUA/MCH = 1:4) method by about three orders of magnitude. The achieved good sensitivity may be attributed to the synergistic effect between GO and AuNPs in this study. In addition, GO could immobilize more antibodies due to the abundant carboxylic groups on its surface. Furthermore, we also demonstrated that the results from this sensor have good reproducibility, with coefficients of variation (CVs) < 8% for IgG. Therefore, the present strategy provides a novel and convenient method for chemical and biochemical quantification and determination. |
| Databáze: | OpenAIRE |
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