Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay
| Autor: | Kazuyuki Sogawa, Yoshiko Sugita-Konishi, Akinori Shimada, Yui Shibata, Yoshio Kodera, Taiji Nakano, Mamoru Satoh, Yuria Takahashi, Naoki Shimojo, Fumiya Yamaide, Fumio Nomura, Kazuhiko Iwahashi, Toshio Tanaka, Hironori Sato |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male food.ingredient Immunology Immunoblotting Biology Immunoglobulin E medicine.disease_cause Andrology 03 medical and health sciences 0302 clinical medicine food Blood serum Allergen Food allergy Yolk medicine Immunology and Allergy Humans Child Egg Hypersensitivity chemistry.chemical_classification Infant General Medicine Allergens medicine.disease Egg Yolk 030104 developmental biology 030228 respiratory system chemistry Egg allergy Child Preschool embryonic structures biology.protein Female Glycoprotein Egg white |
| Zdroj: | International archives of allergy and immunology. 176(3-4) |
| ISSN: | 1423-0097 |
| Popis: | Background: Food allergy is a serious health issue affecting roughly 4% of children, with a substantial effect on quality of life. Chicken egg allergy is frequently observed in infants. Therefore, some of them have to exclude hen’s eggs from their daily diet to avoid allergenic symptoms. Hen’s egg is composed of 2 soluble parts; one is egg white, which has been characterized as the major source of allergenicity, while the other is egg yolk, which is estimated as a miner source. Only 2 allergens from egg yolk, α-livetin (Gal d 5) and YGP42 (Gal d 6), have been described to date. Methods: Sera from 53 patients allergic to hen’s eggs and 2 patients allergic to sesame were obtained from the Department of Pediatrics, Chiba University Hospital. The study was performed using SDS-PAGE, IgE immunoblotting, and dot blotting. Results: Seven bands of egg yolk were detected by IgE immunoblotting. Out of these bands, a possible new allergen was further characterized by LC-MS/MS. The 33-kDa band was identified as yolk glycoprotein (YGP40) by LC-MS/MS. A total of 21 of the 53 patients (47%) had YGP40 detected by dot blotting. Conclusions: We identified YGP40 as a new hen’s egg yolk allergen and detected 4 sites of YGP40 as linear epitopes. |
| Databáze: | OpenAIRE |
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