Extracellular vesicles released by mesenchymal-like prostate carcinoma cells modulate EMT state of recipient epithelial-like carcinoma cells through regulation of AR signaling
| Autor: | Alexandre de la Taille, Pascale Maillé, Guido Jenster, Virginie Firlej, Damien Destouches, Stéphane Terry, Ihsan Y. El-Sayed, Francis Vacherot, Enis Kostallari, Ahmad Daher, Raghida Abou Merhi, Nathaline Haidar-Ahmad, Eric Huet |
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| Přispěvatelé: | Urology |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cancer Research Epithelial-Mesenchymal Transition Neoplasms Hormone-Dependent Time Factors Antineoplastic Agents Hormonal Biology Transforming Growth Factor beta1 03 medical and health sciences Prostate cancer chemistry.chemical_compound 0302 clinical medicine Cell Movement Cell-Derived Microparticles Cell Line Tumor medicine Carcinoma Tumor Microenvironment Enzalutamide Humans Neoplasm Invasiveness Dose-Response Relationship Drug Mesenchymal stem cell Cancer Prostatic Neoplasms Androgen Antagonists medicine.disease Androgen receptor 030104 developmental biology Phenotype Oncology chemistry Drug Resistance Neoplasm Receptors Androgen 030220 oncology & carcinogenesis Immunology Cancer cell Cancer research Signal transduction Signal Transduction |
| Zdroj: | Cancer Letters, 410, 100-111. Elsevier Ireland Ltd |
| ISSN: | 0304-3835 |
| Popis: | Extracellular vesicles released from cancer cells may play an important role in cancer progression by shuttling oncogenic information into recipient cells. However, our knowledge is still fragmentary and there remain numerous questions regarding the mechanisms at play and the functional consequences of these interactions. We have recently established a mesenchymal-like prostate cancer cell line (22Rv1/CR-1; Mes-PCa). In this study, we assessed the effects of the extracellular vesicles released by these cells on recipient androgen-dependent epithelial VCaP prostate cancer cells. Mes-PCa derived vesicles were found to promote mesenchymal features in the recipient epithelial-like prostate cancer cells. This transformation was accompanied by a modulation of androgen receptor signaling and activation of TGFβ signaling pathway. Moreover, recipient cells acquiring mesenchymal traits displayed enhanced migratory and invasive features as well as increased resistance to the androgen receptor antagonist, enzalutamide. Our results suggest a previously unappreciated role for Mes-PCa secreted vesicles in cancer promotion by transferring cell-mediated signals and promoting phenotypic changes in recipient prostate cancer cells. |
| Databáze: | OpenAIRE |
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