Induction of Necrosis in Human Liver Tumor Cells by α-Phellandrene

Autor:	Yi Chen Li, Lan Chi Hsieh, Shu Ling Hsieh, Jing Gung Chung, Weng Cheng Chang, Chih-Chung Wu
Rok vydání:	2014
Předmět:	Cancer Research Programmed cell death Necrosis Cell Survival Medicine (miscellaneous) Antineoplastic Agents Apoptosis Cyclohexane Monoterpenes Biology Nitric Oxide Nitric oxide chemistry.chemical_compound Adenosine Triphosphate Cell Line Tumor Lactate dehydrogenase medicine Humans Viability assay bcl-2-Associated X Protein Membrane Potential Mitochondrial Nutrition and Dietetics Dose-Response Relationship Drug L-Lactate Dehydrogenase Caspase 3 Cell Cycle Cell cycle Molecular biology Cell biology Oncology chemistry Cell culture Hepatocytes Monoterpenes Poly(ADP-ribose) Polymerases medicine.symptom Reactive Oxygen Species
Zdroj:	Nutrition and Cancer. 66:970-979
ISSN:	1532-7914 0163-5581
DOI:	10.1080/01635581.2014.936946
Popis:	α-Phellandrene (α-PA) is a component of dietary spices and herbs. The effect of α-PA on anticancer is unclear. This study aims to investigate the effects of α-PA on liver tumor cell death fate. Human liver tumor (J5) cells were incubated with α-PA and analyzed for cell cycle distribution, expression of Bax, Bcl-2, poly (ADP-ribose) polymerase (PARP) protein, and caspase-3 activity of J5 cells, and levels of nitric oxide (NO) production, lactate dehydrogenase (LDH) leakage, and ATP depletion were also analyzed in this study. Results found that α-PA significantly (P < 0.05) decreased the cell viability of J5 cells after 24-h treatment. The cell cycle distribution, Bax, Bcl-2, PARP protein levels, and caspase-3 activity of J5 cells did not change for 24 h after treatment with 30 μM α-PA. Reactive oxygen species levels significantly increased, mitochondrial membrane potential levels significantly decreased when J5 cells were treated with 30 μM α-PA for 24 h (P < 0.05). Thirty μM α-PA significantly (P < 0.05) increased the necrotic cell number, NO production, LDH leakage, and ATP depletion after 24 h of incubation. These results suggest that α-PA induced J5 cell necrosis but not apoptosis, and α-PA-induced necrosis possibly involved ATP depletion.
Databáze:	OpenAIRE
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