Activity against Mycobacterium tuberculosis with concomitant induction of cellular immune responses by a tetraaza-macrocycle with acetate pendant arms
| Autor: | Rita Delgado, Judite Costa, Suzana David, Diane J. Ordway, Maria-Jorge Arroz |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male T-Lymphocytes medicine.medical_treatment CD14 Population Antitubercular Agents Colony Count Microbial Apoptosis Pilot Projects Lymphocyte Activation Heterocyclic Compounds 2-Ring Microbiology Mycobacterium tuberculosis Interferon-gamma Immune system Annexin medicine Humans Tuberculosis Interferon gamma Annexin A5 education Molecular Biology education.field_of_study biology Macrophages Drug Resistance Microbial General Medicine Immunotherapy biology.organism_classification Leukocyte Common Antigens Female Interleukin-5 Cell Division medicine.drug |
| Zdroj: | Research in Microbiology. 152:569-576 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/s0923-2508(01)01231-1 |
| Popis: | The novel tetraaza-macrocyclic compound 3,7,11-tris(carboxymethyl)-3,7,11,17-tetraaza-bicyclo[11.3.1]hep tadeca-1(17),13,15-triene, abbreviated as ac 3 py14, was investigated for its activity against Mycobacterium tuberculosis and for induction of protective cellular immune responses. Perspective results show that ac 3 py14 and its Fe 3+ 1:1 complex, [Fe(ac 3 py14)], inhibited radiometric growth of several strains of M. tuberculosis . Inhibition with 25 μg/mL varied from 99% for H37Rv to 80% and above for multiple drug-resistant clinical isolates. The capacity of ac 3 py14 to elicit a beneficial immune response without cellular apoptosis was assessed and compared to the effects of virulent M. tuberculosis . The present study produces evidence that after stimulation with ac 3 py14 there was significant production of interferon gamma (IFN- γ ), whereas the production of interleukin-5 (IL-5) remained low, and there was development of a memory population (CD45RO). The level of binding of Annexin V, a marker of apoptosis, was not sufficient to result in toxic effects toward αβ and γδ T cells and CD14 + macrophages. This preliminary study is the first report of a compound that simultaneously exerts an inhibitory effect against M. tuberculosis and induces factors associated with protective immune responses. |
| Databáze: | OpenAIRE |
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