The production and evaluation of monoclonal antibodies to Clostridium perfringens type D epsilon toxin
| Autor: | M.G. Sojka, V.J. White, C.D.H. Boarer, P.L. Roeder |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Clostridium perfringens
medicine.drug_class Bacterial Toxins Blotting Western Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody medicine.disease_cause General Biochemistry Genetics and Molecular Biology Epitope Microbiology Mice medicine Animals Clostridiaceae Pathogen Chromatography High Pressure Liquid Mice Inbred BALB C Hybridomas Toxin Antibodies Monoclonal biology.organism_classification Trypsinization Blot Antibody Formation Chromatography Gel Female |
| Zdroj: | Journal of Biological Standardization. 16:207-218 |
| ISSN: | 0092-1157 |
| DOI: | 10.1016/0092-1157(88)90008-x |
| Popis: | The production of five monoclonal antibodies to the epsilon prototoxin of Clostridium perfringens is described. All five monoclonal antibodies located three proteins in a trypsinized preparation of epsilon prototoxin. These proteins were located at 37.6 kDal, 35.6 kDal and 33.7 kDal by Western blots. Two of the monoclonal antibodies, M26/2 and M27/12, neutralized epsilon toxin in the mouse lethality assay. Four of the five monoclonal antibodies are considered suitable as reagents to detect epsilon toxin in assay procedures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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