Phase locking and multiple oscillating attractors for the coupled mammalian clock and cell cycle
| Autor: | Shoko Saito, Mike J. Downey, Céline Feillet, Patrick Martin, Filippo Tamanini, Michèle Teboul, Francis Lévi, Gijsbertus T. J. van der Horst, Franck Delaunay, Roel C. Janssens, Peter Krusche, Till Bretschneider, David A. Rand |
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| Přispěvatelé: | Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Molecular Genetics |
| Rok vydání: | 2014 |
| Předmět: |
Cell division
Period (gene) Circadian clock Population CLOCK Proteins Cell Cycle Proteins Biology Dexamethasone coupled oscillators Mice MESH: Cell Cycle Proteins SDG 3 - Good Health and Well-being Animals MESH: Animals MESH: Circadian Rhythm Cell Cycle Protein education Oscillating gene MESH: Mice [SDV.BDD]Life Sciences [q-bio]/Development Biology Communication education.field_of_study Multidisciplinary business.industry Biological Sciences MESH: CLOCK Proteins Bacterial circadian rhythms Circadian Rhythm circadian rhythms MESH: Dexamethasone gating oscillations NIH 3T3 Cells business Neuroscience MESH: NIH 3T3 Cells |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2014, 111 (27), pp.9828-33 Proceedings of the National Academy of Sciences of the U.S.A., 111(27), 9828-9833. National Academy of Sciences |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1320474111 |
| Popis: | International audience; Daily synchronous rhythms of cell division at the tissue or organism level are observed in many species and suggest that the circadian clock and cell cycle oscillators are coupled. For mammals, despite known mechanistic interactions, the effect of such coupling on clock and cell cycle progression, and hence its biological relevance, is not understood. In particular, we do not know how the temporal organization of cell division at the single-cell level produces this daily rhythm at the tissue level. Here we use multispectral imaging of single live cells, computational methods, and mathematical modeling to address this question in proliferating mouse fibroblasts. We show that in unsynchronized cells the cell cycle and circadian clock robustly phase lock each other in a 1:1 fashion so that in an expanding cell population the two oscillators oscillate in a synchronized way with a common frequency. Dexamethasone-induced synchronization reveals additional clock states. As well as the low-period phase-locked state there are distinct coexisting states with a significantly higher period clock. Cells transition to these states after dexamethasone synchronization. The temporal coordination of cell division by phase locking to the clock at a single-cell level has significant implications because disordered circadian function is increasingly being linked to the pathogenesis of many diseases, including cancer. |
| Databáze: | OpenAIRE |
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