DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

Autor: Lily M. L. Ou, Hua-Zhong Yu, Bixia Ge, Zhihui Yao, Ping Li
Jazyk: angličtina
Rok vydání: 2015
Předmět:
endocrine system
Cost effectiveness
hCG
Enzyme-Linked Immunosorbent Assay
Biosensing Techniques
lcsh:Chemical technology
Chorionic Gonadotropin
Sensitivity and Specificity
Biochemistry
Redox
Article
Analytical Chemistry
Human chorionic gonadotropin
chemistry.chemical_compound
Follicle-stimulating hormone
electrochemical immunoassay
Pregnancy
Cations
redox labeling
medicine
Humans
lcsh:TP1-1185
Electrical and Electronic Engineering
Instrumentation
Immunoassay
Detection limit
Chromatography
medicine.diagnostic_test
Reproducibility of Results
Signal Processing
Computer-Assisted
signal enhancement
DNA
Electrochemical Techniques
Molecular biology
Atomic and Molecular Physics
and Optics
chemistry
DNA-cation interaction
Female
Selectivity
Oxidation-Reduction
hormones
hormone substitutes
and hormone antagonists
Zdroj: Sensors, Vol 15, Iss 8, Pp 20543-20556 (2015)
Sensors
Volume 15
Issue 8
Pages 20543-20556
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Popis: A simple DNA-redox cation interaction enhancement strategy has been developed to improve the sensitivity of electrochemical immunosensors for protein detection. Instead of labeling with fluorophores or redox-active groups, the detection antibodies were tethered with DNA single strands. Based on the electrostatic interaction between redox cations ([Ru(NH3)6](3+)) and negatively charged DNA backbone, enhanced electrochemical signals were obtained. Human chorionic gonadotropin (hCG) detection has been performed as a trial analysis. A linear response range up to the concentration of 25 mIU/mL and a detection limit of 1.25 mIU/mL have been achieved, both are comparable with the ultrasensitive enzyme-linked immunosorbent assay (ELISA) tests. The method also shows great selectivity towards hCG over other hormones such as thyroid stimulating hormone (TSH) and follicle stimulating hormone (FSH). By and large, our approach bears the merits of cost effectiveness and simplicity of instrumentation in comparison with conventional optical detection methods.
Databáze: OpenAIRE
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