Ekspresja antygenu Fas i FasL na limfocytach T i B w przerosłych migdałkach gardłowych u dzieci chorych na wysiękowe zapalenie ucha
| Autor: | Małgorzata Mrugacz, Jolanta Wysocka, Janusz Żak, Karol Ratomski, Bożena Skotnicka, Beata Żelazowska-Rutkowska |
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| Rok vydání: | 2008 |
| Předmět: |
biology
wysiękowe zapalenie ucha business.industry flow cytometry apoptosis Germinal center hemic and immune systems chemical and pharmacologic phenomena Fas receptor Adenoid Molecular biology CD19 Fas ligand medicine.anatomical_structure Otorhinolaryngology Antigen Apoptosis biology.protein otitis media with effusion Medicine apoptoza business cytometria przepływowa CD8 |
| Zdroj: | Otolaryngologia Polska. 62:59-64 |
| ISSN: | 0030-6657 |
| DOI: | 10.1016/s0030-6657(08)70210-5 |
| Popis: | Summary Introduction Apoptosis of lymphocytes can be induced by different factors mainly however the activation of antigen Fas (CD95) and his ligand FasL (CD95L) route. Apoptosis induced by Fas-FasL has large significance in elimination of lymphocytes T in final phase immune response designate AICD (activation induced cell death). Lymphocytes B undergo apoptosis induced by Fas-FasL in germinal center, which has special meaning in elimination of cells of low specificity and weak affinity of receptor BCR (B-cell antigen receptor). Aim of study The aim of this study was evaluation of percentage of apoptotic lymphocytes and expression of Fas and FasL in CD4+, CD8+, CD19+ lymphocytes in hypertrophied adenoid and hypertrophied adenoid and otitis media with effusion. Methods CD4+ Fas+, CD8+Fas+, CD19+Fas+ CD4+ FasL+, CD8+FasL+, CD19+FasL+ cells subpopulation were identified using monoclonal antibodies and flow cytometry method. Results The percentage of lymphocytes CD4+ Fas+, CD8+Fas+, CD19+Fas+ was higher in hypertrophied adenoid and otitis media with effusion compared to the control group. There was no significant difference the percentage CD4+ Fas+, CD8+Fas+, CD19+Fas+ CD4+ FasL+, CD8+FasL+, CD19+FasL+ in hypertrophied adenoid and otitis media with effusion than the control group. The percentage of apoptotic lymphocytes was higher in hypertrophied adenoid and otitis media with effusion compared to the control group. Conclusions The susceptibility the individual subpopulation of lymphocytes in hypertrophied adenoid seems to influence on change of relations of quantitative lymphocytes and upset the immunological function of tonsils which can influence on course otitis media with effusion at children. |
| Databáze: | OpenAIRE |
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