NMDA receptor stimulation induces temporary a-tubulin degradation signaled by nitric oxide-mediated tyrosine nitration in the nervous system of Sepia officinalis
| Autor: | Gabriella Fiore, Marco d'Ischia, Anna Di Cosmo, Anna Palumbo, Carlo Di Cristo |
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| Přispěvatelé: | Anna, Palumbo, Gabriella, Fiore, CRISTO CARLO, Di, A., DI COSMO, D'Ischia, M., Palumbo, Anna, Fiore, Gabriella, DI CRISTO, Carlo, DI COSMO, Anna, D'Ischia, Marco |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Nitrogen Blotting Western Biophysics Stimulation Nitric Oxide Biochemistry Nitroarginine Receptors N-Methyl-D-Aspartate Nitric oxide chemistry.chemical_compound Tubulin Detyrosination medicine Animals Sepia Tyrosine Enzyme Inhibitors Molecular Biology biology Nervous tissue Optic Lobe Nonmammalian Cell Biology Immunohistochemistry Precipitin Tests Cell biology Nitric oxide synthase medicine.anatomical_structure chemistry Mollusca biology.protein Electrophoresis Polyacrylamide Gel Signal Transduction |
| Popis: | Biochemical and immunohistochemical evidence is reported, showing basal protein nitration in specific regions of the optic lobes of Sepia officinalis, mainly in the fiber layers of the plexiform zone. SDS-PAGE analysis of optic lobe extracts revealed an intense 3-nitrotyrosine immunoreactive band identified as alpha-tubulin by immunoprecipitation and partial purification. Stimulation of NMDA receptors resulted in a selective decrease in alpha-tubulin levels within 30 min with partial recovery after 4 h. The effect was suppressed by the NO synthase (NOS) inhibitor L-nitroarginine. Incubation of optic lobes with free 3-nitrotyrosine resulted likewise in a selective loss of alpha-tubulin, due apparently to incorporation of the amino acid into the C-terminus of detyrosinated alpha-tubulin to give the nitrated protein purportedly more susceptible to degradation. Overall, these results point to a novel potential physiologic role of NO and free 3-nitrotyrosine in the control of the alpha-tubulin tyrosination/detyrosination cycle and turnover in Sepia nervous tissue. |
| Databáze: | OpenAIRE |
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