Yuehchukene, a Bis-Indole Alkaloid, and Cyclophosphamide Are Active in Breast Cancer in vitro
| Autor: | T.W.T. Leung, T.C.C. Poon, C.H. Chui, J.K.J. Tjong, F.Y. Lau, Johnny Cheuk On Tang, G. Cheng, S.K.W. Ho, K.F. Cheng, Y.C. Kong, W.C.P. Tse |
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| Rok vydání: | 1999 |
| Předmět: |
Yuehchukene
medicine.medical_specialty Cyclophosphamide medicine.drug_class Tetrazolium Salts Breast Neoplasms Pharmacology chemistry.chemical_compound Alkaloids Internal medicine Antineoplastic Combined Chemotherapy Protocols Drug Discovery Tumor Cells Cultured medicine Humans Cytotoxic T cell Pharmacology (medical) Antineoplastic Agents Alkylating Reverse Transcriptase Polymerase Chain Reaction Cell growth business.industry Alkaloid Drug Synergism General Medicine Nitrogen mustard Thiazoles Infectious Diseases Endocrinology Oncology chemistry Cell culture Estrogen Drug Screening Assays Antitumor business medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1421-9794 0009-3157 |
| DOI: | 10.1159/000007257 |
| Popis: | Yuehchukene (YCK) is a novel bis-indole alkaloid with weak estrogenic activity. Biochemical studies showed that YCK could attenuate estrogenic action. In this study, the response of MCF-7, an estrogen-receptor-positive breast cancer cell line, under different combinations of estradiol, cyclophosphamide and YCK, was tested. From the dose-response curve, we discovered that 10–2 M cyclophosphamide, even in its so-called ‘bio-inert’ form, could inhibit MCF-7 cell growth. However, the cytotoxic effect of cyclophosphamide was lost by reducing its concentration to ∼1 × 10–3 M. On the other hand, a low concentration (∼10–8–10–9 M) of YCK was found to potentiate the cytotoxic effect of cyclophosphamide on the MCF-7 cell line. Such an effect was absent in the estrogen-receptor-negative cell line MDA-MB-231. These findings, together with the dual role of a mixed estrogen and anti-estrogen effect, suggested that YCK and cyclophosphamide can be a potential combination in chemo-hormonal therapy for breast cancer. |
| Databáze: | OpenAIRE |
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