Determination of albendazole sulfoxide in human plasma by using liquid chromatography-tandem mass spectrometry
| Autor: | Ezgi Fikirdesici, Güler Yağmur Özkan, Erkin Alkan, Onursal Sağlam, Nihan Burul-Bozkurt, Nihal Saraner, Berrak Güney, Mevlüt Yıldırım |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Electrospray ionization 030106 microbiology Clinical Biochemistry Mass spectrometry Albendazole 01 natural sciences Biochemistry Sensitivity and Specificity Analytical Chemistry Ion 03 medical and health sciences chemistry.chemical_compound Liquid chromatography–mass spectrometry Tandem Mass Spectrometry medicine Protein precipitation Humans Chromatography 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Albendazole sulfoxide chemistry Linear Models Methanol medicine.drug Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1022 |
| ISSN: | 1873-376X |
| Popis: | A rapid, simple and sensitive method was developed and validated using liquid chromatography–tandem mass spectrometry (LC–MS/MS) for determination of albendazole sulfoxide (ABZOX) in human plasma. The plasma samples were extracted by protein precipitation using albendazole sulfoxide-d3 as internal standard (IS). The chromatographic separation was performed on Waters Xbridge C18Column (100 × 4.6 mm, 3.5 μm) with a mobile phase consisting of ammonia solution, water and methanol at a flow rate of 0.70 mL/min. ABZOX was detected and identified by mass spectrometry with electrospray ionization (ESI) in positive ion and multiple-reaction monitoring (MRM) mode. The method was linear in the range of 3–1500 ng/mL for ABZOX. This method was successfully applied to the bioequivalence study in human plasma samples. |
| Databáze: | OpenAIRE |
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