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Context: In patients usingsaliva substitute (SS) which would be a suitable aesthetic restorative material. Aims: To evaluate and compare the effect of saliva substitute on the microhardness of direct tooth coloured restorative materials. Settings and Design:This is an in vitro study for evaluation of Vickers microhardness (VM) where standard uniform disc‑shaped specimens were immersed in a saliva substitute (SS) for 30 days. Methods and Material: The tooth‑colored restorative materials tested in this study were Group I- resin modified glass ionomer cement (RM-GIC), Group II-light cured fluoride releasing restorative material (Ketac N100) and Group III-light cured resin composite restorative material (Filtek Z 350 XT).Forty‑eight disc‑shaped samples, 16 of each material were prepared. Samples belonging to respective groups were randomly divided into two subgroups with 8 samples each. Subgroup A was immersed in distilled water (DW) and subgroup B in saliva substitute (SS). In addition 3 samples of group I, II and III were prepared for scanning electron microscopic examination. VM value were calculated from the table of ISO 6507 standard. Pre and post-immersion microhardness measurements were made on the same surface of all samples. Statistical Analysis Used: Normality of data obtained was tested using Kolmogorov-Smirnov and Shapiro-Wilk test, then parametric tests were used to compare the means between different groups. The level of significance was set at 5% and the p values less than 0.05 was considered statistically significant. Results: All the restorative materials when immersed in SS showed statistically significant reduction (p |