Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 2: Application to the analysis of calcitonin containing pharmaceutical formulation

Autor: Sreekumar Kurungot, Szilveszter Gáspár, Mohamed Salah Medjram, Sabrina Lamraoui, Rabah Boukherroub, Sorin Melinte, Alina Vasilescu, Santosh K. Singh, Roxana Jijie, Ran Ye, Sabine Szunerits, Samia Boulahneche
Přispěvatelé: International Centre of Biodynamics, Université Catholique de Louvain = Catholic University of Louvain (UCL), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire de Génie Chimique et Environnement, Université 20 Août 1955 Skikda, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), UCL - SST/ICTM/ELEN - Pôle en ingénierie électrique
Rok vydání: 2018
Předmět:
Zdroj: Electrochimica Acta
Electrochimica Acta, 2018, 266, pp.364-372. ⟨10.1016/j.electacta.2018.02.038⟩
Electrochimica Acta, no.266, p. 364-372 (2018)
Electrochimica Acta, Elsevier, 2018, 266, pp.364-372. ⟨10.1016/j.electacta.2018.02.038⟩
ISSN: 0013-4686
DOI: 10.1016/j.electacta.2018.02.038
Popis: In part 1 (A. Vasilescu et al., Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 1: Lysozyme aggregation at pH 2 and 7.4 Electrochem. Acta, 254 (2017) 375–383) we proposed porous reduced graphene oxide coated glassy carbon electrode (GC/prGO) in combination with differential pulse voltammetry as a new analytical tool for aggregation studies of proteins. Lysozyme was used as a model to follow its aggregation by electrochemical means at pH 2 and pH 7.4, leading to the formation of amyloid and amorphous aggregates, respectively. Part 2 of this work widens the scope of this approach by investigating a biopharmaceutical product, as the aggregation of peptide based drugs affects their therapeutic activity and can induce allergic reactions in patients. The salmon polypeptide calcitonin (sCT) was chosen as an example of a bioactive peptide with limited pharmaceutical potential due to a tendency to form cytotoxic aggregates and amyloid fibrils. For practical applications, screen printed electrodes (SPE) and flexible electrodes (FE) modified with polydiallyldimethylammonium (PDDA) and prGO by using the layer-by-layer deposition technique have been developed for the detection of sCT. The results indicate that these electrodes can differentiate between formation of amyloid aggregates of calcitonin (2 mg mL−1) in citrate buffer to no aggregation in acetate buffer. It was further demonstrated that these electrodes are able to analyze a pharmaceutical drug product of low potency, Miacalcic (8.3 μg mL−1), where no aggregation was observed.
Databáze: OpenAIRE
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