Steroid specificity of the putative DHB receptor: evidence that the receptor is not 11βHSD
| Autor: | Karen Khoo, Karen E. Sheppard, Zygmunt S. Krozowski, Kevin X. Z. Li |
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| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Colon Physiology Endocrinology Diabetes and Metabolism CHO Cells Spironolactone Biology Transfection Substrate Specificity Rats Sprague-Dawley Glucocorticoid receptor Mineralocorticoid receptor Cricetinae Physiology (medical) Internal medicine medicine Animals Intestinal Mucosa Binding site Receptor Cells Cultured Cell Nucleus Chinese hamster ovary cell Hydroxysteroid Dehydrogenases Recombinant Proteins Rats Endocrinology Nuclear receptor Biochemistry Culture Media Conditioned Nuclear receptor coactivator 3 Carbenoxolone 11-beta-Hydroxysteroid Dehydrogenases Estrogen-related receptor gamma Corticosterone |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 275:E124-E131 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1998.275.1.e124 |
| Popis: | Recently, we identified a novel putative nuclear receptor in colonic crypt cells distinct from both mineralocorticoid receptor and glucocorticoid receptor, with high affinity for 11-dehydrocorticosterone (11-DHB) (33). In the present study, competitive nuclear binding assays demonstrated that this site has a unique steroid binding specificity that distinguishes it from other steroid receptors. Western blot analysis showed the presence of 11β-hydroxysteroid dehydrogenase-2 (11βHSD2) but not 11βHSD1in colonic crypt cells and showed that 11βHSD2was present in the nuclear pellet. Differences in steroid specificity between the putative DHB receptor and inhibition of 11βHSD activity indicate that binding is not to the enzyme. Furthermore, modified Chinese hamster ovary cells transfected with the 11βHSD2gene express nuclear 11βHSD2but not a nuclear DHB binding site. In conclusion, these data support the existence of a novel nuclear DHB receptor in rat colon that is distinct from the classic steroid receptors and from both 11βHSD1and 11βHSD2. |
| Databáze: | OpenAIRE |
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