Virologic, immunologic, and clinical evaluation of human immunodeficiency virus antibody status of symptom-free children born to infected mothers
Autor: | Victor Israell, Kang-Niam Liu, Joanna Dobrosyzcki, Jim Conroy, Andrew Wiznia, Genevieve Lambert, Jonathan W. M. Gold, Maura Porricolo, Penny Baron, Nicholas Chelyapov, Philip A. Brunell |
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Rok vydání: | 1994 |
Předmět: |
CD4-Positive T-Lymphocytes
Adolescent Population Viremia HIV Infections HIV Antibodies Asymptomatic Polymerase Chain Reaction Cohort Studies Leukocyte Count Acquired immunodeficiency syndrome (AIDS) Pregnancy Immunopathology HIV Seronegativity medicine Humans Pregnancy Complications Infectious education Sida Child education.field_of_study biology business.industry Infant Newborn virus diseases Infant medicine.disease biology.organism_classification Immunoglobulin A Immunoglobulin M Child Preschool Immunoglobulin G Pediatrics Perinatology and Child Health Immunology Cohort DNA Viral HIV-1 Female Viral disease medicine.symptom business T-Lymphocytes Cytotoxic |
Zdroj: | The Journal of pediatrics. 125(3) |
ISSN: | 0022-3476 |
Popis: | Study objective: To determine the prevalence of infection by the human immunodeficiency virus (HIV) in a population of symptom-free children who were born to HIV-infected mothers and who subsequently underwent seroreversion from an HIV antibody-positive to an HIV antibody-negative status. Design Cohort. Setting Pediatric HIV program in a community setting. Patients We used HIV DNA polymerase chain reaction (PCR) and coculture to detect the presence or absence of HIV in peripheral blood mononuclear cells of 134 children aged 6 to 53 months. All children had HIV antibody at birth and underwent a subsequent seroreversion to antibody-negative status. Results In 134 children with HIV antibody-negative status, 219 of 220 culture results and 242 of 247 HIV-1 DNA PCR assay results were negative. Six positive laboratory results were obtained for six different children, each of whom had negative results on multiple assays. For HIV-infected children, 56 of 62 cultures and 99 of 104 PCR evaluations showed positive results. There was no clinical or laboratory evidence of HIV infection in the group with HIV antibody-negative status. Conclusion We were unable to find evidence of latent HIV type 1 infection in this cohort of symptom-free children who underwent seroreversion to HIV antibody-negative status. The loss of maternal HIV antibody in these children indicates the absence of HIV infection. False-positive PCR and culture results occurred sporadically, indicating that repeated analysis of HIV seropositivity in infants and children is necessary. |
Databáze: | OpenAIRE |
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