Ca2+ entry through L-type voltage-sensitive Ca2+ channels stimulates the release of human chorionic gonadotrophin and placental lactogen by placental explants
| Autor: | Philippe Lebrun, Bruno Polliotti, Claude Robyn, Sylvain Meuris |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
endocrine system
medicine.medical_specialty Nifedipine Placenta chemistry.chemical_element Calcium Biology Chorionic Gonadotropin Organ Culture Techniques Internal medicine medicine Extracellular Humans Secretion Gallopamil Placental lactogen Molecular Biology Calcium channel Cell Biology 3-Pyridinecarboxylic acid 1 4-dihydro-2 6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)- Methyl ester Placental Lactogen Up-Regulation medicine.anatomical_structure Endocrinology chemistry Potassium Calcium Channels medicine.drug Explant culture |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1220:101-106 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(94)90124-4 |
| Popis: | The release of human chorionic gonadotrophin (hCG) and placental lactogen (hPL) by human placental explants can be stimulated by Ca2+ entry. The aim of the present study was to characterize the modality of Ca2+ entry in the presence of high extracellular K+ concentration ([K+]o). A rise in [K+]o from 5 to > or = 50 mM induced a rapid and marked increase in the release of hCG and hPL from human term placental explants. The stimulatory effects of an excess [K+]o on the release of hCG and hPL were blocked in the absence of extracellular Ca2+ or in the presence of 0.5 mM Co2+. The presence of 50 microM methoxyverapamil, 20 microM nifedipine or 40 microM Cd2+ in the medium inhibited the stimulatory effects of [K+]o addition. Lastly, 40 microM Ni2+ failed to affect the increases in hCG and hPL releases elicited by [K+]o addition. Our data clearly show that a rise in [K+]o stimulates the release of hCG and hPL from placental explants. These secretory effects can be viewed as resulting from a Ca2+ entry through voltage-sensitive Ca2+ channels of the L-type. |
| Databáze: | OpenAIRE |
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