Identification and Expression of a Gene Encoding an Epitope that Induces Hemagglutination Inhibition Antibody to Avibacterium paragallinarum Serovar A
| Autor: | Taichi Noro, Kazuhiko Yaguchi, Katsuhiko Amimoto, Eiji Oishi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.drug_class
Monoclonal antibody Epitope Antibodies law.invention Epitopes Mice Food Animals Western blot law medicine Animals Cloning Molecular Gene Antigens Bacterial Mice Inbred BALB C Hemagglutination assay General Immunology and Microbiology biology medicine.diagnostic_test Immunogenicity Hemagglutination Antibodies Monoclonal Building and Construction Hemagglutination Inhibition Tests Virology Molecular biology Specific Pathogen-Free Organisms Gene Expression Regulation Bacterial Vaccines Recombinant DNA biology.protein Animal Science and Zoology Female Antibody Pasteurellaceae Pasteurellaceae Infections Chickens |
| Zdroj: | Avian Diseases Digest. 2:e13-e13 |
| ISSN: | 1933-5334 |
| DOI: | 10.1637/1933-5334(2007)2[e13:iaeoag]2.0.co;2 |
| Popis: | The aims of this study were the identification, cloning, and expression of a genetic region encoding an epitope that induces hemagglutination inhibition (HI) antibody against Avibacterium paragallinarum serovar A and an evaluation of the recombinant protein for immunogenicity in chickens. Although two monoclonal antibodies (MAbs) with HI activity, designated S24-951 and S7-1716-5C, were generated in this study, no reactive proteins with both MAbs were identified by Western blot analysis. A gene fragment of 5157 bp, designated hpa5. 1, was cloned from genomic DNA, and a recombinant protein expressed by hpa5.1, designated HPA5.1, reacted with both MAbs on dot-blot analysis. HPA5.1 showed no hemagglutinating activity, but significantly absorbed HI antibodies in the chicken immune serum. Analysis using a series of deletion mutants prepared from hpa5.1 indicated that a 4.8 kbp gene in hpa5.1 is essential for the expression epitope recognized by MAb S24-951. In addition, chickens immunized once with HPA5.1 showed a high protection rate with sufficient HI antibody titers against challenge exposure with a virulent strain of A. paragallinarum serovar A strain 221. These results show that hpa5. I1 is responsible for the expression of an epitope that induces HI antibody, and HPA5.1 might be a candidate for the development of a new vaccine against avian infectious coryza caused by A. paragallinarum serovar A. |
| Databáze: | OpenAIRE |
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