Effect of gluten diet on blood innate immune gene expressions and stool consistency in Spix's Saddleback Tamarin (Leontocebus fuscicollis) raised in captivity
| Autor: | Evonnildo Costa Gonçalves, Michael A. Huffman, Taianara Tocantins Gomes Almeida, Alexandre do Rosário Casseb, Maria Vivina Barros Monteiro, Frederico Ozanan Barros Monteiro, Rafaelle Casseb Guimarães, Ednaldo da Silva Filho |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Diarrhea beta-Defensins Glutens Captivity Physiology Gene Expression digestive system 03 medical and health sciences Diet Gluten-Free Feces 0302 clinical medicine Plant matter Genetics Animals Molecular Biology Gene Stool consistency chemistry.chemical_classification Inflammation Innate immune system biology nutritional and metabolic diseases Tamarin General Medicine biology.organism_classification Gluten digestive system diseases Immunity Innate Toll-Like Receptor 5 030104 developmental biology chemistry 030220 oncology & carcinogenesis Callitrichinae |
| Zdroj: | Molecular biology reports. 46(4) |
| ISSN: | 1573-4978 |
| Popis: | The callitrichids are non-human primates that feed on insects and plant matter in nature, but in captivity, they are fed mostly an artificial diet containing amounts of gluten, in their toxic forms in items such as wheat, barley and rye. The aim of this research was to estimate the blood β-defensin and Toll like receptor 5 (TLR5) gene expressions and to analyze the stool consistency (firm, soft, diarrheic) in Leontocebus fuscicollis raised in captivity. Blood samples of animals under gluten-free and gluten diets were collected and their fecal output quality was periodically monitored and classified during the course of the study. Gene expression was evaluated using real-time PCR. The stool consistencies of individuals fed a gluten diet were most frequently soft or diarrheic, while it was mostly normal in individuals fed a gluten-free diet. β-Defensin expression increased in individuals fed a gluten diet, but decreased after 15 days. Expression normalized between 30 and 45 days on a gluten-free diet. However, expression of the TLR5 gene did not change under a gluten diet. A gluten diet affects stool quality, and brings about an immediate increase in blood β-defensin expression in the beginning but decreases after 15 days. |
| Databáze: | OpenAIRE |
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