Growth characteristics of porcine chlamydial strains in different cell culture systems and comparison with ovine and avian chlamydial strains
| Autor: | Irene Schiller, Petra Gysling, Andreas Pospischil, Andrea Schifferli |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Swine
Cell Culture Techniques Chlamydiaceae Infections Cycloheximide Microbiology chemistry.chemical_compound Chlamydia suis Chlorocebus aethiops Animals Humans Chlamydiaceae Chlamydia Columbidae Apical cytoplasm Vero Cells Swine Diseases Chlamydophila Sheep General Veterinary biology biology.organism_classification chemistry Cell culture Vero cell Animal Science and Zoology Caco-2 Cells Intracellular |
| Zdroj: | The Veterinary Journal. 168:74-80 |
| ISSN: | 1090-0233 |
| DOI: | 10.1016/s1090-0233(03)00039-x |
| Popis: | Porcine Chlamydiaceae were cultivated under various culture conditions and we compared their growth characteristics with those of ruminant and avian strains. The combination of centrifugation assisted cell culture infection and cycloheximide treatment of Vero cell coverslip cultures provided the highest inclusion numbers with all chlamydial strains. Interestingly, the use of Iscove's modified Dulbecco's medium instead of Eagle's minimal essential medium significantly increased Chlamydia suis inclusion counts. C. suis and Chlamydophila pecorum inclusion numbers were markedly increased in CaCo cells, compared with Vero cells. This accelerated growth of porcine Chlamydiaceae under certain cultivation conditions may be helpful for the propagation of low chlamydial numbers or for their isolation from field samples. The intracellular distribution of porcine Chlamydiaceae in polarised CaCo cells clearly demonstrated differences between the chlamydial strains: C. pecorum 1710S inclusions were predominantly localised in the apical cytoplasm, C. suis S45 inclusions, however, were mostly situated in lower cytoplasmatic compartments. These findings might reflect biological differences in vivo. |
| Databáze: | OpenAIRE |
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