Morin exhibits leukemic cellular apoptosis through caspase pathway
| Autor: | Marcelo Rocha da Costa, Wander Lopes Pereira, Liovando Marciano da Costa, Marcelo Augusto Filardi, Tânia Toledo de Oliveira, Milton M. Kanashiro |
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| Rok vydání: | 2020 |
| Předmět: |
Programmed cell death
Apoptosis Plant Science Morin 01 natural sciences Biochemistry Analytical Chemistry Flow cytometry chemistry.chemical_compound Cell Line Tumor medicine Humans Inner mitochondrial membrane Caspase Flavonoids Membrane Potential Mitochondrial Membrane potential medicine.diagnostic_test biology Caspase 3 010405 organic chemistry Organic Chemistry Molecular biology 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Caspases Cancer cell biology.protein |
| Zdroj: | Natural Product Research. 35:5554-5558 |
| ISSN: | 1478-6427 1478-6419 |
| Popis: | The present study investigated the possibility of apoptosis-inducing activity in human leukemia U-937 and THP-1 cells by the flavonoid morin. The treatments were evaluated by using the MTT and LDH assays; analysis of mitochondrial membrane potential (ΔΨm) was evaluated by flow cytometry, cell death by apoptosis was confirmed by fluorescence microscopy and by assessing the activity of caspases-3 and −6. The data indicated that the flavonoid morin has promoted a decrease in cell viability in a concentration-dependent way for both of the cancerous cell lines. An increase in the percentage of cell death caused by apoptosis was associated to a potential alteration in the mitochondrial membrane (ΔΨm) suggesting the involvement of cell death in intrinsic apoptotic pathways. Activation of caspases-3 and −6 confirmed the presence of apoptotic activity from morin. The results reinforce the antileukemic potential of flavonol morin. |
| Databáze: | OpenAIRE |
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