Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: a multicenter prospective study of 2302 patients in China
| Autor: | Zhuogang Liu, Yongrong Lai, Hui Sun, Daobin Zhou, Xin Wang, Yingmin Liang, Juan Li, Zefeng Xu, Huanling Zhu, Yin Zhang, Ping Zou, Xie-Qun Chen, Zong-Hong Shao, Yan Li, Fangping Chen, Jin Zhou, Xiao-Jun Huang, Ming Hou, Chun Wang, Guang-sen Zhang, Hai Bai, Wei Li, Lian-Sheng Zhang, Xin Du, Yu-hong Zhou, Jin-Ying Lin, Lin Qiu, Depei Wu, Ming Jiang, Xiaomin Wang, Yue-Yun Lai, Mei-Yun Fang |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty China Adolescent Biology Young Adult Predictive Value of Tests Cytogenetic Abnormality medicine Humans Prospective Studies Prospective cohort study Child In Situ Hybridization Fluorescence Aged Fluorescent Dyes Aged 80 and over Chromosome Aberrations medicine.diagnostic_test Myelodysplastic syndromes Cytogenetics Fish analysis Hematology Middle Aged Reference Standards medicine.disease Oncology Myelodysplastic Syndromes Cytogenetic Analysis Fish Female Fluorescence in situ hybridization |
| Zdroj: | Leukemia research. 39(5) |
| ISSN: | 1873-5835 |
| Popis: | In an attempt to establish the advantages of fluorescence in situ hybridization (FISH) studies over conventional cytogenetic (CC) analysis, a total of 2302 de novo MDS patients from 31 Chinese institutions were prospectively selected in the present study for both CC and standardized FISH analysis for +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities. CC analysis was successful in 94.0% of the patients; of these patients, 35.9% of the cases were abnormal. FISH analysis was successful in all 2302 patients and detected at least one type of common cytogenetic abnormality in 42.7% of the cases. The incidences of +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities by FISH were 4.1% to 8.7% higher than those by CC. FISH identified abnormalities in 23.6% of the patients exhibiting normal CC results and revealed that 20.7% of the patients with adequate normal metaphases (≥20) had abnormal clones. FISH identified cytogenetic abnormalities in 50.4% of the patients with failed CC analysis. In summary, our multicenter studies emphasised and confirmed the importance of applying standardized FISH testing based on an appropriate panel of probes to detect common cytogenetic abnormalities in Chinese de novo MDS patients, particularly those with normal or failed CC results. |
| Databáze: | OpenAIRE |
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