Effect of Alpha-Linolenic Acid with Bovine Serum Albumin or Methyl-Beta-Cyclodextrin on Membrane Integrity and Oxidative Stress of Frozen-Thawed Boar Sperm
| Autor: | Hee-Tae Cheong, Choon-Keun Park, Wook-Hwan Kim, Won-Hee Lee, Boo-Keun Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Cryopreservation Reactive oxygen species biology alpha-Linolenic acid Acrosomal membrane medicine.disease_cause Sperm Lipid peroxidation Andrology Original Research Paper chemistry.chemical_compound Bovine serum albumin chemistry Oxidative stress Methyle-beta-cyclodextrin medicine biology.protein Boar sperm Alpha-linolenic acid Sperm motility Membrane potential |
| Zdroj: | Development & Reproduction |
| ISSN: | 2465-9541 2465-9525 |
| Popis: | The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively). To confirm the effect of two carrier proteins, same volume of BSA and MBCD without ALA were added during cryopreservation. Membrane damage, mitochondrial activity, reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were measured using flow cytometry, and movement of sperm tail as motility parameter and morphological abnormality were observed under light microscope. In results, all of sperm parameters were enhanced by ALA combined with BSA or MBCD compared to control groups (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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