Pathways Related to NLRP3 Inflammasome Activation Induced by Gold Nanorods

Autor: Rob J. Vandebriel, Sylvie Remy, Jolanda P. Vermeulen, Evelien G. E. Hurkmans, Kirsten Kevenaar, Neus G. Bastús, Beatriz Pelaz, Mahmoud G. Soliman, Victor F. Puntes, Wolfgang J. Parak, Jeroen L. A. Pennings, Inge Nelissen
Přispěvatelé: Institut Català de la Salut, [Vandebriel RJ, Vermeulen JP, Hurkmans EGE, Kevenaar K] Centre for Health Protection, National Institute for Public Health & the Environment, Bilthoven, The Netherlands. [Remy S] Health Unit, VITO NV, Mol, Belgium. [Bastús NG] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Universitat Autònoma de Barcelona, Bellaterra, Spain. [Puntes VF] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Universitat Autònoma de Barcelona, Bellaterra, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, European Commission
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Macromolecular Substances::Multiprotein Complexes::Inflammasomes [CHEMICALS AND DRUGS]
Inflammasomes
Technology
Industry
and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Metal Nanoparticles [TECHNOLOGY
INDUSTRY
AND AGRICULTURE]
oxidative phosphorylation
Metal Nanoparticles
Or
Nanorod
purinergic receptor
tecnología
industria y agricultura::productos manufacturados::nanoestructuras::nanotubos [TECNOLOGÍA
INDUSTRIA Y AGRICULTURA]
Catalysis
tecnología
industria y agricultura::productos manufacturados::nanoestructuras::nanopartículas::nanopartículas metálicas [TECNOLOGÍA
INDUSTRIA Y AGRICULTURA]
Inorganic Chemistry
NLRP3
NLR Family
Pyrin Domain-Containing 3 Protein
Humans
Oxidative phosphorylation
Physical and Theoretical Chemistry
paraoxonase-2
Molecular Biology
Nanosphere
Paraoxonase-2
Spectroscopy
Technology
Industry
and Agriculture::Manufactured Materials::Nanostructures::Nanotubes [TECHNOLOGY
INDUSTRY
AND AGRICULTURE]
Inflammation
Nanotubes
sustancias macromoleculares::complejos multiproteicos::inflamasomas [COMPUESTOS QUÍMICOS Y DROGAS]
Nanopartícules
integumentary system
Organic Chemistry
cholesterol
General Medicine
gold
nanorod
Computer Science Applications
nanosphere
Cholesterol
inflammation
nanostar
Gold
Nanotubs
Purinergic receptor
Nanostar
Zdroj: Scientia
Digital.CSIC. Repositorio Institucional del CSIC
instname
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5763
Popis: The widespread and increasing use of engineered nanomaterials (ENM) increases the risk of human exposure, generating concern that ENM may provoke adverse health effects. In this respect, their physicochemical characteristics are critical. The immune system may respond to ENM through inflammatory reactions. The NLRP3 inflammasome responds to a wide range of ENM, and its activation is associated with various inflammatory diseases. Recently, anisotropic ENM have become of increasing interest, but knowledge of their effects on the immune system is still limited. The objective of the study was to compare the effects of gold ENM of different shapes on NLRP3 inflammasome activation and related signalling pathways. Differentiated THP-1 cells (wildtype, ASC-or NLRP3-deficient), were exposed to PEGylated gold nanorods, nanostars, and nanospheres, and, thus, also different surface chemistries, to assess NLRP3 inflammasome activation. Next, the exposed cells were subjected to gene expression analysis. Nanorods, but not nanostars or nanospheres, showed NLRP3 inflammasome activation. ASC-or NLRP3-deficient cells did not show this effect. Gene Set Enrichment Analysis revealed that gold nanorod-induced NLRP3 inflammasome activation was accompanied by downregulated sterol/cholesterol biosynthesis, oxidative phosphorylation, and purinergic receptor signalling. At the level of individual genes, downregulation of Paraoxonase-2, a protein that controls oxidative stress, was most notable. In conclusion, the shape and surface chemistry of gold nanoparticles determine NLRP3 inflammasome activation. Future studies should include particle uptake and intracellular localization.
This research was funded by the EU FP7 project FutureNanoNeeds (GA N° 604602), RIVM, and VITO.
Databáze: OpenAIRE
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