Comparative analysis of the effects of collection methods on salivary steroids
| Autor: | Xiangyu Sun, Shuguo Zheng, Chao Yuan, Fangqiao Wei, Qidi Ren, Ce Zhu, Shunlan Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Saliva Concentration Secretion rate medicine.medical_treatment Physiology Dehydroepiandrosterone Stimulation Flow rate 03 medical and health sciences 0302 clinical medicine Medicine Humans Testosterone General Dentistry Collection methods Salivary steroids business.industry Research Comparative analysis RK1-715 030206 dentistry Salivary flow rate Steroid hormone 030104 developmental biology Dentistry Mastication Steroids business Secretory Rate |
| Zdroj: | BMC Oral Health BMC Oral Health, Vol 21, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1472-6831 |
| Popis: | Background Steroid hormone test for saliva was a promising area of research, however the impact of different collection methods on salivary steroids was underexplored so far. This study was designed to compare the effects of different collection methods (unstimulated or stimulated by chewing paraffin, forepart or midstream) on salivary flow rate, concentrations and secretion rates of steroids in saliva. Methods Whole-saliva samples were collected from 10 systemically and orally healthy participants, whose forepart and midstream segments of saliva were collected under unstimulated and stimulated conditions, with the salivary flow rate of each sample recorded. The concentrations and secretion rates of salivary steroids including testosterone, dehydroepiandrosterone (DHEA) and progesterone were measured by ELISA, with the multiple of change calculated. Results The results indicated mechanical stimulation used in collection of saliva samples could affect concentrations and secretion rates of steroids, whereas forepart and midstream segments had little differences in levels of salivary steroids, which effects could be partly influenced by individual specificity. The asynchronism in change of secretion rate of steroids with that of salivary flow rate might play an important role during this course. Conclusion Based on these findings, we suggested to use the same collection method throughout one analytical study on salivary steroids or in longitudinal observations to ensure the comparability of the saliva samples collected. |
| Databáze: | OpenAIRE |
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