Changes in Gene and Protein Expression in Magnetic Field–Treated Human Glioma Cells

Autor:	W. H. Hanneman, E. M. Hennessey, W. G. Lotz, Mary Helen Kanitz, Frank A. Witzmann, D. Conover, Russell E. Savage
Rok vydání:	2005
Předmět:	Human glioma Microarray Health Toxicology and Mutagenesis Cancer Biology Toxicology medicine.disease Proteomics Molecular biology Glioma medicine Gene and protein expression Gene Carcinogen
Zdroj:	Toxicology Mechanisms and Methods. 15:115-120
ISSN:	1537-6524 1537-6516
Popis:	Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 muT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 muT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b6f39de2059f0e97999cc4d588f4f19 https://doi.org/10.1080/15376520590918829 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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