Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson's disease
| Autor: | Yong-Cheng Jiang, Ying Chen, Hai-Ying Zhang, Jia-Nan Yan, Kaifu Ke, Xiao-Su Gu, Jiabing Shen, Jun-Rui Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Autophagosome
autophagy Parkinson's disease alpha-synuclein Neuroprotection chemistry.chemical_compound Developmental Neuroscience Precursor cell medicine RC346-429 Protein kinase B PI3K/AKT/mTOR pathway skin-derived precursor Schwann cells Alpha-synuclein Autophagy medicine.disease Cell biology chemistry neuroprotection Neurology. Diseases of the nervous system autophagosomes neural regeneration parkinson′s disease pi3k/akt/mtor pathway skin-derived precursor schwann cells Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 17, Iss 6, Pp 1357-1363 (2022) |
| ISSN: | 1876-7958 1673-5374 |
| Popis: | Autophagy has been shown to play an important role in Parkinson's disease. We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson's disease through affecting autophagy. In this study, 6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells (SKP-SCs). The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine, reduced excessive autophagy, increased tyrosine hydroxylase expression, reduced α-synuclein expression, reduced the autophagosome number, and activated the PI3K/AKT/mTOR pathway. Autophagy activator rapamycin inhibited the effects of SKP-SCs, and autophagy inhibitor 3-methyladenine had the opposite effect. These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy, thereby exhibiting a neuroprotective effect in a cellular model of Parkinson's disease. This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University (approval No. S20181009-205) on October 9, 2018. |
| Databáze: | OpenAIRE |
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