In utero sister chromatid exchange analysis for detection of transplacental mutagens

Autor:	Gaither D. Bynum, Gerhard C. Senula, Edward L. Schneider, David Kram
Rok vydání:	1979
Předmět:	Fetus Multidisciplinary Fetal dna DNA damage food and beverages Transplacental Sister chromatid exchange Biology Chromatids Molecular biology Mice Teratogens In utero Pregnancy Sister chromatids Animals Pregnancy Animal Female Crossing Over Genetic Metaphase Maternal-Fetal Exchange Mutagens
Zdroj:	Nature. 279(5713)
ISSN:	0028-0836
Popis:	FETAL exposure to agents which damage DNA can result in birth anomalies, cancer and inherited abnormalities. In the present report we describe a new approach for the detection of fetal DNA damage through the enumeration of sister chromatid exchanges (SCE) in mouse fetal chromosomes. SCE can be identified as reciprocal exchanges of fluorescent intensities betweeen sister chromatids in metaphase cells which have divided twice in the presence of bromodeoxyuride (BrdU) (Fig. 1). Analysis of SCE has been shown to be a sensitive and reproducible means of detecting chemical mutagens and carcinogens1–9.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b6cc356bf78fed4d46424cb00c3297e https://pubmed.ncbi.nlm.nih.gov/450096 Zobrazit plný text záznamu